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Yeast Genetics Methods and Protocols 1st Edition by Jeffrey S Smith, Daniel J Burke ISBN 149391362X 9781493913626

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Yeast Genetics Methods and Protocols 1st Edition Jeffrey S. Smith Digital Instant Download

Author(s): Jeffrey S. Smith, Daniel J. Burke (eds.)
ISBN(s): 9781493913626, 149391362X
Edition: 1
File Details: PDF, 8.07 MB
Year: 2014
Language: english
SKU: EB-4765900 Category: Tags: ,

EYeast Genetics Methods and Protocols 1st Edition by Jeffrey S Smith, Daniel J Burke – Ebook PDF Instant Download/Delivery:149391362X, 9781493913626
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Product details: 

ISBN 10: 149391362X 

ISBN 13: 9781493913626

Author: Jeffrey S. Smith, Daniel J. Burke 

Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.

Table of contents: 

  1. Yeast Transformation by LiAc/SS Carrier DNA/PEG Method
  2. Tetrad, Random Spore, and Molecular Analysis of Meiotic Segregation and Recombination
  3. PCR Mutagenesis and Gap Repair in Yeast
  4. PCR-Mediated Epitope Tagging of Genes in Yeast
  5. Manipulating the Yeast Genome: Deletion, Mutation, and Tagging by PCR
  6. Preparation of Yeast Cells for Live Cell Imaging and Indirect Immunofluorescence
  7. Single Yeast Cell Imaging
  8. Microfluidic Platforms for Generating Dynamic Environmental Perturbations to Study the Responses of Single Yeast Cells
  9. Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations
  10. Synthetic Genetic Array Analysis of Global Mapping of Genetic Networks in Yeast
  11. Chemical Genetic and Chemogenomic Analysis in Yeast
  12. Phenomic Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays
  13. Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast
  14. Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast
  15. Global Analysis of Transcription Factor Binding Sites in Yeast Using ChIP-Seq
  16. High-Density Tiling Microarrays Analysis of the Full Transcriptional Activity of Yeast
  17. Analysis of Silencing in Saccharomyces cerevisiae
  18. A User’s Guide to the Ribosomal DNA in Saccharomyces cerevisiae
  19. Two-Dimensional Agarose Gel Electrophoresis for Analysis of DNA Replication
  20. Replicative Life Span Analysis in Budding Yeast
  21. Metabolomic and Lipidomic Analyses of Chronologically Aging Yeast

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Jeffrey S Smith,Genetics,Genetics,Protocols