Post translational Modifications of Proteins Tools for Functional Proteomics 2nd Edition by Christoph Kannicht 9781588297198 1588297195
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ISBN 10: 1588297195
ISBN 13: 9781588297198
Author: Christoph Kannicht
The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition, this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed prools focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology™ series. Each chapter provides a brief introduction to the topic, step-by-step laboratory prools, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting edge, Post-translational Modifications of Proteins: Tools for Functional Proteomics, Second Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.
Table of contents:
Chapter 1 Disulfide Bond Mapping by Cyanylation-Induced Cleavage and Mass Spectrometry
Chapter 2 Detection of Post-Translational Modifications by Fluorescent Staining of Two-Dimensional Gels
Chapter 3 Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods
Chapter 4 Analysis of Tyrosine-O-Sulfation
Chapter 5 Amidated Peptides: Approaches for Analysis
Chapter 6 Glutamate and Hydroxyaspartate in Proteins
Chapter 7 Lysine Hydroxylation and Cross-Linking of Collagen
Chapter 8 Mass Spectrometric Determination of Protein Ubiquitination
Chapter 9 Analysis of Sumoylation
Chapter 10 Detection and Analysis of Protein ISGylation
Chapter 11 Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins
Chapter 12 Analysis of S-Acylation of Proteins
Chapter 13 Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa
Chapter 14 Two-Dimensional Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern
Chapter 15 Carbohydrate Composition Analysis of Glycoproteins by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag
Chapter 16 Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB)
Chapter 17 Separation of N-Glycans by HPLC
Chapter 18 Enzymatic Sequence Analysis of N-Glycans by Exoglycosidase Cleavage and Mass Spectrometry – Detection of Lewis X Structures
Chapter 19 Immunochemical Methods for the Rapid Screening of the O-Glycosidically Linked N-Acetylglucosamine Modification of Proteins
Chapter 20 Analysis of O-Glycosylation
Chapter 21 Characterization of Site-Specific N-Glycosylation
Chapter 22 Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency by HPLC Using Highly Fluorescent Anthranilic Acid (AA) Tag
Chapter 23 Comparability and Monitoring Immunogenic N-Linked Oligosaccharides from Recombinant Monoclonal Antibodies from Two Different Cell Lines Using HPLC with Fluorescence Detection and Mass Spectrometry
Chapter 24 Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N-Linked Oligosaccharides of Recombinant Monoclonal Antibodies
Chapter 25 Web-Based Computational Tools for the Prediction and Analysis of Post-Translational Modifications of Proteins
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Tags: Christoph Kannicht, translational, Modifications, Proteins