Lectins Analytical Technologies 1st Edition by Carol L Nilsson ISBN 0444530770 9780444530776

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ISBN 10: 0444530770 
ISBN 13: 9780444530776
Author: Carol L Nilsson 

Lectins: Analytical Technologies covers both analytical and biological aspects of lectins (functional carbohydrate (complex sugar) recognition proteins) and provides researchers in the field with a resource containing background information and ‘look-up’ tables detailing lectin specificity and structures. Also included are methods and practical tips for designing new lectins from existing non-lectin proteins, automated approaches to lectin proteomics and high resolution mass spectrometry techniques.

This book will be of interest to both novice and advanced researchers in biomedical, analytical and pharmaceutical fields who are involved in the study of lectin structures or who utilize lectins as analytical tools. The study of lectins and their employment in analytical settings spans a range of fields including:
* Crystallography and lectin structure databases
* Carbohydrate microarrays for lectin characterization and glycotope identification
* Proteomic approaches to the functional identification of bacterial adhesins
* Generation of lectins from enzymes
* Probing cell-surface lectins with neoglycoconjugates

* Reviews up-to-date techniques, including practical hints for laboratory work
* Provides overview of lectin e-resources and several color illustrations
* Includes a ‘look-up’ table detailing lectin specificity

Lectins Analytical Technologies 1st Table of contents:

Chapter 1. Lectins: Analytical Tools from Nature
1. Introduction
2. A Short History of Lectins
3. Lectin Structure and Function
4. Lectins as Analytical Tools
5. Lectin Resources
Acknowledgments
References
Chapter 2. Crystallography and Lectin Structure Database
1. Introduction
2. X-Ray Crystallography
3. Lectin Databases
4. Recent Research Highlights
5. Hands-on Protocols and Practical Tips
References
Chapter 3. NMR Investigations of Lectin–Carbohydrate Interactions
1. Introduction
2. Protein Expression and Purification
3. Fast or Slow Exchange
4. How to Track Chemical Shift Changes
5. How to Obtain 3D Structures of Lectin–Carbohydrate Complexes
6. Transferred NMR Methods
7. Emerging NMR Methodologies
8. Summary
References
Chapter 4. Applications of Isothermal Titration Calorimetry to Lectin–Carbohydrate Interactions
1. Introduction
2. Characterization of the Binding Specificity and Binding Sites of Lectins
3. Binding of Multivalent Carbohydrates to Lectins
Acknowledgments
References
Chapter 5. Bioanalytical Studies Based on Lectin–Carbohydrate Interactions Measured by Ellipsometr
1. Introduction
2. Ellipsometry
References
Chapter 6. Use of Natural and Synthetic Oligosaccharide, Neoglycolipid and Glycolipid Libraries in D
1. Introduction
2. Analytical Technologies for Analysis of Carbohydrate Pathogen Binding
3. Carbohydrate Libraries
4. Libraries in Analysis of Novel H. pylori Binding Specificities
5. Poly-N-Acetyllactosamine Receptors for Influenza Virus
References
Chapter 7. Carbohydrate Microarrays for Lectin Characterization and Glyco-Epitope Identification
1. Introduction
2. The Specificity-Based Lectin Classification
3. Carbohydrate Microarrays
4. Exploring Lectin-Binding Profiles Using Carbohydrate Microarrays
References
Chapter 8. Glycoproteomics Based on Lectin Affinity Chromatographic Selection of Glycoforms
1. Proteomic Strategies
2. Recognizing Post-Translational Modifications
3. Lectin Selectors
4. Serial Lectin Affinity Chromatography
5. Proteomic Strategies Based on Lectin Selection
6. The Future
References
Chapter 9. Miniaturized Lectin Affinity Chromatography
1. Introduction
2. Principle of Miniaturized LAC
3. Preparation and Applications of Miniaturized LAC
4. Perspective
Acknowledgment
References
Chapter 10. Frontal Affinity Chromatography: Systematization for Quantitative Interaction Analysis B
1. Introduction
2. General Features and Principle
3. Operation
4.Applications
5. Concluding Remarks
References
Chapter 11. Technical Aspects of Glycoprotein Enrichment
1. Introduction
2. Practical Aspects of Lectin Affinity Chromatography
3. Lectins Immobilized to Agarose Gels
4. Lectins Immobilized to Porous Silica
5. Lectins Immobilized to Monoliths
6. Lectins Immobilized to Other Materials
7. Enrichment with Hydrazide-Activated Particles
8. Conclusions
References
Chapter 12. Proteomic Techniques for Functional Identification of Bacterial Adhesins
1. Introduction
2. Protein Separation Techniques
3. Biological Mass Spectrometry
4. Bacterial Adhesins and Virulence
5. Proteomic Analysis of H. pylori
6. Functional Identification a Lactoferrin-Binding Protein of H. pylori
Acknowledgments
References
Chapter 13. The Use of Lectins in Bioaffinity MALDI Probes
1. Introduction and Background
2. A General Method for Producing Bioaffinity MALDI Probes
3. Identification and Structure Elucidation of Lectin-Binding Oligosaccharides by Bioaffinity MALDI
References
Chapter 14. Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for
1. Introduction
2. ESI FT-ICR MS Instrumentation
3. FT-ICR MS Operating Principles
4. Performance of FT-ICR MS
5. Top-Down and Bottom-Up Proteomics
6. Tandem Mass Spectrometry in ESI FT-ICR MS
7. Negative Ion Mode Tandem Mass Spectrometry
8. Metal-Assisted Tandem Mass Spectrometry
9. Summary
Acknowledgments
References
Chapter 15. Catalytically Inactive Endoglycosidases as Microbial Diagnostic Reagents: Chitinases and
1. Introduction
2. Use of E35Q HEWL for Bacterial Diagnostics
References
Chapter 16. Generation of Lectins from Enzymes: Use of Inactive Endosialidase for Polysialic Acid De
1. Polysialic Acid
2. Endosialidase-Green Fluorescent Protein Fusion Construct
3.Applications of the Fusion Protein
4. Comments
5. Experimental Considerations
References
Chapter 17. Probing Cell Surface Lectins with Neoglycoconjugates
1. Introduction
2. Design of Neoglycoconjugates as Probes
3. Experimental Approaches
4. Examples of Application
5. Discussion

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