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MicroRNA Protocols 2nd Edition by Shao Yao Ying 1627030824 9781627030823

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MicroRNA Protocols 2nd Edition Shao-Yao Ying Digital Instant Download

Author(s): Shao-Yao Ying, Donald C. Chang, Shi-Lung Lin (auth.), Shao-Yao Ying (eds.)
ISBN(s): 9781627030823, 1627030824
Edition: 2
File Details: PDF, 7.35 MB
Year: 2013
Language: english
SKU: EB-4318928 Category: Tags: , , ,

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Product details:

ISBN 10: 1627030824 

ISBN 13: 9781627030823

Author: Shao Yao Ying

Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity; yet many questions still remain.  In MicroRNA Protocols, Second Edition, experts in the field provide up-to-date coverage of this diverse area of study.  The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and known pitfalls.

Fully updated and easy to use, MicroRNA Protocols, Second Edition aims to stimulate readers to explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields.

Table of contents:

  1. The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions

  2. Structure Analysis of MicroRNA Precursors

  3. MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex

  4. Recognition and Cleavage of Primary MicroRNA Transcripts

  5. Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery

  6. MicroRNAs and Messenger RNA Turnover

  7. Prediction of MicroRNA Targets

  8. Prediction of Human MicroRNA Targets

  9. Complications in Mammalian MicroRNA Target Prediction

  10. miRBase: The MicroRNA Sequence Database

  11. Methodologies for High-Throughput Expression Profiling of MicroRNAs

  12. In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development

  13. Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA

  14. Cloning MicroRNAs From Mammalian Tissues

  15. Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation

  16. Identifying MicroRNA Regulators of Cell Death in Drosophila

  17. MicroRNAs in Human Immunodeficiency Virus-1 Infection

  18. Cloning and Detection of HIV-1-Encoded MicroRNA

  19. Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein

  20. In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates

  21. Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector

  22. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs

  23. Isolation and Identification of Gene-Specific MicroRNAs

  24. Transgene-Like Animal Models Using Intronic MicroRNAs

  25. Evolution of MicroRNAs

  26. Perspectives

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Tags: Shao Yao Ying, MicroRNA, Protocols