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Nucleic Acid and Peptide Aptamers Methods and Protocols 1st Edition by Günter Mayer 1934115894 9781934115893

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Nucleic Acid and Peptide Aptamers Methods and Protocols 1st Edition Shawn K. Piasecki Digital Instant Download

Author(s): Shawn K. Piasecki, Bradley Hall, Andrew D. Ellington (auth.), Günter Mayer (eds.)
ISBN(s): 9781934115893, 1934115894
Edition: 1
File Details: PDF, 5.47 MB
Year: 2009
Language: english
SKU: EB-1315654 Category: Tags: , , ,

Nucleic Acid and Peptide Aptamers Methods and Protocols 1st Edition by Günter Mayer – Ebook PDF Instant Download/Delivery: 1934115894, 9781934115893

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Product details:

ISBN 10: 1934115894 

ISBN 13: 9781934115893

Author: Günter Mayer

After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In “Nucleic Acid and Peptide Aptamers: Methods and Protocols”, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, “Nucleic Acid and Peptide Aptamers: Methods and Protocols” will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.

Table of contents:

  1. Nucleic Acid Pool Preparation and Characterization

  2. In Vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins

  3. Isolating Aptamers Using Capillary Electrophoresis-SELEX (CE-SELEX)

  4. In Vitro Selection of Allosteric Ribozymes

  5. Cell-Specific Aptamers for Targeted Therapies

  6. Aptamers Targeting RNA Molecules

  7. Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction

  8. Structural Probing Techniques on Natural Aptamers

  9. Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography

  10. Locked Nucleic Acid Aptamers

  11. Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay

  12. Aptamers as Artificial Gene Regulation Elements

  13. Aptamers and Biosensors

  14. Nanoparticles/Dip Stick

  15. In Vivo Imaging of Oligonucleotidic Aptamers

  16. Selection of Peptides Interfering with Protein-Protein Interaction

  17. In Vitro Selection of Protein and Peptide Libraries Using mRNA Display

  18. Ligand-Regulated Peptide Aptamers

  19. Isolation of Peptide Aptamers to Target Protein Function

  20. Microbodies

  21. Peptide Aptamers for Small Molecule Drug Discovery

  22. Synthesis and Application of Peptides as Drug Carriers

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Tags: Günter Mayer, Nucleic, Peptide, Aptamers, Methods