Laboratory Exercises in Organismal and Molecular Microbiology 1st Edition by Steve Alexander, Dennis Strete, Mary Jane Niles 0072487445 9780072487442

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Product details:

ISBN 10: 0072487445 

ISBN 13: 9780072487442

Author: Steve K. Alexander, Dennis Strete, Mary Jane Niles

A modern general microbiology laboratory manual that combines the procedural details of a laboratory manual with the photographic support of a laboratory atlas. The 46 class-tested laboratory experiments are divided into 9 specialty areas, and the extensive four-color illustration program includes 220 photos and micrographs plus 150 line drawings.

Table of contents:

1. Structure, Function, and Use of the Microscope

2. Micro. Comparisons of Microorganisms, Multi. Parasites & Micro. Invert

3. Microbial Procaryotes: Bacteria and Cyanobacteria

4. Microbial Eucaryotes: Fungi

5. Microbial Eucaryotes: Protozoans and Algae

6. Flatworms and Roundworms

7. Zooplankton

8. Disease Vectors

9. Negative Stain

10. Smear Preparation and the Simple Stain

11. Gram Stain

12. Acid-fast Stain

13. Spore Stain

14. Capsule and Flagella Stains

15. The Staining Characterization of a Bacterial Unknown

16. Bacteria & Fungi in the Lab. Environ: The Necessity of Aseptic Technique

17. Preparation and Inoculation of Growth Media

18. Culture Characterization of Bacteria

19. Biochemical Tests Used to Identify Bacteria

20. Application: Identification of a Nonclinical Bacterial Unknown

21. Isolation and Identification of Staphylococci from the Skin

22. Isolation and Identification of Streptococci from the Throat

23. Iden. of Ent. Bacteria, Enterotubes, a Rapid System to Iden. Ent. Bacteria

24. Isolation and Identification of Bacteria from the Urinary Tract

25. Assessing Antibiotic Effectiveness: The Kirby-Bauer Method

26. Identification of a Clinical Bacterial Unknown

27. Killing Bacteria with High Temperature

28. Skin Disinfection: Evaluating Antiseptics and Hand Sanitizers

29. Cleaning Countertops with Disinfectants

30. Bacteriological Examination of Drinking Water Using the MPN Method

31. Bacterial DNA Isolation and Southern Analysis

32. Mutagenesis in Bacteria: The Ames Test

33. Plasmid Isolation and Restriction Mapping

34. Acquiring Antibiotic Resistance Through Bacterial Transformation

35. Acquiring Antibiotic Resistance Through Bacterial Conjugation

36. Viral DNA Fingerprinting

37. Isolation of Bacteriophage from Sewage and Determination of Phage Titer

38. The Virus Infection Cycle: The One-step Growth Curve

39. Infection of Plant Leaves with Tobacco Mosaic Virus

40. Identification and Enumeration of White Blood Cells

41. Antigen-Antibody Precipitation Reactions and Determination of Antibody Titer

42. Agglutination Reactions: ABO Blood Typing

43. Immunodiffusion: Antigen-Antibody Precipitation Reactions in Gels

44. Enzyme-linked Immunosorbent Assay (ELISA)

45. Bacterial Protein Fingerprinting and Western Blotting

46. The Neutralization of Viruses by Antibodies

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Tags: Steve Alexander, Dennis Strete, Mary Jane Niles, Laboratory