Serial Analysis of Gene Expression SAGE Methods and Protocols 1st Edition by Annabeth Laursen Høgh, Kåre Lehmann Nielsen ISBN 1597454540 9781597454544

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Product details: 

ISBN 10: 1597454540 

ISBN 13: 9781597454544

Author: Annabeth Laursen Høgh, Kåre Lehmann Nielsen

Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles.

Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data.

Table of contents: 

Part I: Introduction to SAGE

Part II: Experimental Methods
4. Laboratory Setup and Reagent Preparation
5. cDNA Library Construction for SAGE
6. Tag Extraction and Ligation Strategies
7. Cloning and Sequencing of SAGE Libraries
8. Quality Control and Troubleshooting in SAGE Experiments

Part III: Data Analysis and Interpretation
9. Bioinformatics Tools for SAGE Data Analysis
10. Normalization and Statistical Methods in SAGE
11. Comparative Transcriptomics Using SAGE
12. Visualization and Functional Annotation of SAGE Tags

Part IV: Advanced Applications and Future Perspectives
13. Modifications of SAGE: LongSAGE, SuperSAGE, and DeepSAGE
14. Integrating SAGE with Next-Generation Sequencing (NGS)
15. Future Trends in Gene Expression Profiling

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Annabeth Laursen Høgh,Kåre Lehmann Nielsen,Analysis