Neural Development Methods and Protocols 1st Edition by Renping Zhou, Lin Mei 1627034439 9781627034432
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ISBN 10: 1627034439
ISBN 13: 9781627034432
Author: Renping Zhou, Lin Mei
Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Prools, experts in the field contribute commonly used prools to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, prools for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and accessible, Neural Development: Methods and Prools provides key guidance for students and postdoctoral fellows new to developmental neurobiology.
Table of contents:
Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro
Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture
Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny
Culture of Dissociated Hippocampal Neurons
Culturing Mouse Cerebellar Granule Neurons
Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain
Culturing Astrocytes from Postnatal Rats
Culturing Oligodendrocyte Lineage Cells from Neonatal Rats
Isolation and Culture of Schwann Cells
DNA Transfection: Calcium Phosphate Method
The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices
Lentiviral Vector Production, Titration, and Transduction of Primary Neurons
In-Ovo Electroporation in Embryonic Chick Spinal Cords
Gene Transfer in Developing Chick Embryos: In Ovo Electroporation
In Utero Electroporation in Mice
General Introduction to In Situ Hybridization Protocol Using Non-Radioactively Labeled Probes to Detect mRNAs on Tissue Sections
Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections
β-Galactosidase Staining of LacZ Fusion Proteins in Whole Tissue Preparations
Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei
In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System
Growth Cone Collapse Assay
The Stripe Assay: Studying Growth Preference and Axon Guidance on Binary Choice Substrates In Vitro
Microcontact Printing of Substrate-Bound Protein Patterns for Cell- and Tissue Culture
Semi-Automated Analysis of Dendrite Morphology in Cell Culture
Monitoring Synaptic Plasticity by Imaging AMPA Receptor Content and Dynamics on Dendritic Spines
Morphological Analysis of Neuromuscular Junctions by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms
Routine Histology Techniques for the Developing and Adult Central Nervous System
Cryosectioning
The Golgi-Cox Method
Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine
Retrograde Tracing Technique for Neonatal Animals
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Tags: Renping Zhou, Lin Mei, Neural, Development, Methods