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Cell Senescence Methods and Protocols 2013th Edition by Lorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Guido Kroemer 9781627032384 162703238X

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Cell Senescence Methods and Protocols 2013th Edition Lorenzo Galluzzi Digital Instant Download

Author(s): Lorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Guido Kroemer
ISBN(s): 9781627032384, 162703238X
Edition: 2013
File Details: PDF, 7.79 MB
Year: 2013
Language: english
SKU: EB-4183144 Category: Tags: , , ,

Cell Senescence Methods and Protocols 2013th Edition by Lorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Guido Kroemer – Ebook PDF Instant Download/Delivery: 9781627032384, 162703238X

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Product details:

ISBN 10: 162703238X

ISBN 13: 9781627032384

Author: Lorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Guido Kroemer

Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods thatare now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level andinclude protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Table of contents:

  1. Cell Senescence as Both a Dynamic and a Static Phenotype

  2. Senescence Regulation by mTOR

  3. Senescence Regulation by the p53 Protein Family

  4. Markers of Cellular Senescence

  5. Biomarkers of Cell Senescence Assessed by Imaging Cytometry

  6. Cytofluorometric Assessment of Cell Cycle Progression

  7. Quantification of Cell Cycle-Arresting Proteins

  8. Colorimetric Detection of Senescence-Associated β Galactosidase

  9. Chemiluminescent Detection of Senescence-Associated β Galactosidase

  10. Detection of the Senescence-Associated Secretory Phenotype (SASP)

  11. Unbiased Characterization of the Senescence-Associated Secretome Using SILAC-Based Quantitative Proteomics

  12. Detection of Senescence-Associated Heterochromatin Foci (SAHF)

  13. Monitoring DNA Damage During Cell Senescence

  14. Assessment and Quantification of Telomerase Enzyme Activity

  15. Methods for the Assessment of Telomere Status

  16. Detection of Nuclear Envelope Alterations in Senescence

  17. Measuring Reactive Oxygen Species in Senescent Cells

  18. Quantification of Protein Carbonylation

  19. Assays for the Measurement of Lipid Peroxidation

  20. Raman Spectroscopy for the Detection of AGEs/ALEs

  21. Monitoring Oncogenic B-RAF-Induced Senescence in Melanocytes

  22. Methods to Investigate the Role of SIRT1 in Endothelial Senescence

  23. Monitoring Nutrient Signaling Through the Longevity Protein p66SHC1

  24. Profiling the Metabolic Signature of Senescence

  25. Genome-Wide RNAi Screening to Identify Regulators of Oncogene-Induced Cellular Senescence

  26. An Integrated Approach for Monitoring Cell Senescence

  27. Robust Multiparametric Assessment of Cellular Senescence

  28. Assessing Chronological Aging in Bacteria

  29. Assessing Organismal Aging in the Filamentous Fungus Podospora anserina

  30. Assessing Chronological Aging in Saccharomyces cerevisiae

  31. Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis

  32. High-Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditis elegans

  33. Assessing Senescence in Drosophila Using Video Tracking

  34. Assessing Vascular Senescence in Zebrafish

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Tags: Lorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Guido Kroemer, Senescence