Cell Biology Assays 1st Edition by Geri Kreitzer, Fanny Jaulin, Cedric Espenel ISBN 0123756928 9780123756923

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ISBN 10: 0123756928 
ISBN 13: 9780123756923
Author: Geri Kreitzer, Fanny Jaulin, Cedric Espenel 

Protein assay methods are used for protein identification with blood groups, cell surface markers, drugs and toxins. This text features comprehensive protocols essential for researchers studying various areas of biological and medical sciences. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a vareity of technologies and model systems.

Focus on protein identification using mass spectrometry
Step-by-step procedures detailing materials, procedures, comment and pitfalls
Information on the plethora of technologies needed to tackle complex problems

Cell Biology Assays 1st Table of contents:

Chapter 1. Protein Determination
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Chapter 2. Phosphopeptide Mapping – A Basic Protocol
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Chapter 3. Radioiodination of Proteins and Peptides
Chapter 4. Protein Detection in Gels by Silver Staining – A Procedure Compatible with Mass Spectrome
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Chapter 5. Fluorescence Detection of Proteins in Gels Using SYPRO Dyes
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Chapter 6. Autoradiography and Fluorography – Film-Based Techniques for Imaging Radioactivity in Fla
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Chapter 7. Free-Flow Electrophoresis
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Chapter 8. Gel-Based Proteomics – High-Resolution Two-Dimensional Gel Electrophoresis of Proteins. I
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Chapter 9. High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients for Proteom
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Chapter 10. Two-Dimensional Difference Gel Electrophoresis – Application for the Analysis of Differe
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Chapter 11. Image Analysis and Quantitation
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Chapter 12. Two-Dimensional Gel Profiling of Posttranslationally Modified Proteins by in vivo Isotop
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Chapter 13. Immunoprecipitation of Proteins under Nondenaturing Conditions
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Chapter 14. Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein Intera
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Chapter 15. Analysis of Protein–Protein Interactions by Chemical Cross-Linking
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Chapter 16. Peroxisomal Targeting as a Tool to Assess Protein–Protein Interactions
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Chapter 17. Biomolecular Interaction Analysis Mass Spectrometry
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Chapter 18. Ligand Blot Overlay Assay – Detection of Ca +2 – and Small GTP-Binding Proteins
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Chapter 19. Modular Scale Yeast Two-Hybrid Screening
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Chapter 20. Affinity Electrophoresis for Studies of Biospecific Interactions – High-Resolution Two-D
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Chapter 21. Methods in Protein Ubiquitination
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Chapter 22. Gel Mobility Shift Assay
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Chapter 23. DNA Affinity Chromatography of Transcription Factors – The Oligonucleotide Trapping Appr
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Chapter 24. Protein Identification and Sequencing by Mass Spectrometry
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Chapter 25. Proteome Specific Sample Preparation Methods for Matrix-Assisted Laser Desorption/Ioniza
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Chapter 26. In-Gel Digestion of Protein Spots for Mass Spectrometry
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Chapter 27. Peptide Sequencing by Tandem Mass Spectrometry
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Chapter 28. Direct Database Searching Using Tandem Mass Spectra of Peptides
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Chapter 29. Identification of Proteins from Organisms with Unsequenced Genomes by Tandem Mass Spectr
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Chapter 30. Identification of Protein Phosphorylation Sites by Mass Spectrometry
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Chapter 31. Analysis of Carbohydrates/Glycoproteins by Mass Spectrometry
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Chapter 32. Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics
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Chapter 33. Site-Specific, Stable Isotope Labeling of Cysteinyl Peptides in Complex Peptide Mixtures
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Chapter 34. Protein Hydrogen Exchange Measured by Electrospray Ionization Mass Spectrometry
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Chapter 35. Nongel-Based Proteomics – Selective Reversed-Phase Chromatographic Isolation of Methioni
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Chapter 36. Mass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies
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Tags: Geri Kreitzer, Fanny Jaulin, Cedric Espenel, Cell, Biology