Proteomics of Biological Systems Protein Phosphorylation Using Mass Spectrometry Techniques 1st Edition by Bryan Ham 1118137035 9781118137031

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Product details:

ISBN 10: 1118137035 

ISBN 13: 9781118137031

Author: Bryan M. Ham

Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.

Table of contents:

1. Posttranslational Modification (PTM) of Proteins

2. Glycosylation of Proteins

3. Sulfation of Proteins as Posttranslational Modification

4. Eukaryote PTM as Phosphorylation: Normal State Studies

5. Eukaryote PTM as Phosphorylation: Perturbed State Studies

6. Prokaryotic Phosphorylation of Serine, Threonine, and Tyrosine

7. Prokaryotic Phosphorylation of Histidine

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Tags: Bryan Ham, Proteomics, Biological, Systems, Protein