Sale!

Recombinant Protein Production in Yeast Methods and Protocols 2012th Edition by Roslyn Bill 9781617797699 1617797693

Original price was: $50.00.Current price is: $35.00.

Recombinant Protein Production in Yeast Methods and Protocols 2012th Edition Roslyn M. Bill Digital Instant Download

Author(s): Roslyn M. Bill
ISBN(s): 9781617797699, 1617797693
Edition: 2012
File Details: PDF, 3.18 MB
Year: 2012
Language: english
SKU: EB-2625054 Category: Tag:

Recombinant Protein Production in Yeast Methods and Protocols 2012th Edition by Roslyn Bill – Ebook PDF Instant Download/Delivery: 9781617797699, 1617797693

Full download Recombinant Protein Production in Yeast Methods and Protocols 2012th Edition after payment

 

Product details:

ISBN 10: 1617797693

ISBN 13: 9781617797699

Author: Roslyn M. Bill

In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.

Table of contents:

Chapter 1. Optimising Yeast as a Host for Recombinant Protein Production (review)
Chapter 2. Which Yeast Species Shall I Choose? Saccharomyces cerevisiae versus Pichia pastoris (review)
Chapter 3. Preparation of Pichia pastoris Expression Plasmids
Chapter 4. Preparation of Saccharomyces cerevisiae Expression Plasmids
Chapter 5. Codon Optimisation for Heterologous Gene Expression in Yeast
Chapter 6. Yeast Transformation to Generate High-yielding Clones
Chapter 7. Screening for High-yielding Pichia pastoris Clones: the Production of G Protein-Coupled Receptors as a Case Study
Chapter 8. Screening for High-yielding Saccharomyces cerevisiae Clones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
Chapter 9. The Effect of Antifoam Addition on Protein Production Yields
Chapter 10. Setting up a Bioreactor for Recombinant Protein Production in Yeast
Chapter 11. The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (review)
Chapter 12. On-line Analysis and Process Control in Recombinant Protein Production (review)
Chapter 13. Monitoring the Biomass Accumulation of Recombinant Yeast Cultures: Off-line Estimations of Dry Cell Mass and Cell Counts
Chapter 14. On-line Monitoring of Biomass Accumulation in Recombinant Yeast Cultures
Chapter 15. Optimising P. pastoris Induction
Chapter 16. Optimizing S. cerevisiae Induction Regimes
Chapter 17. Large Scale Production of Membrane Proteins in Pichia pastoris: The Production of G Protein-coupled Receptors as a Case Study
Chapter 18. Large-scale Production of Membrane Proteins in Saccharomyces cerevisiae: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
Chapter 19. Large Scale Production of Secreted Proteins in Pichia pastoris
Chapter 20. Disruption of Yeast Cells to Isolate Recombinant Proteins

People also search:

what is recombinant protein production

yeast recombinant protein expression

yeast protein production

recombinant yeast

recombinant protein production in mammalian cells

Tags: Roslyn Bill, Recombinant, Protein, Production, Methods