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Molecular Methods for Evolutionary Genetics 1st Edition by Virginie Orgogozo, Matthew Rockman 1617792284 9781617792281

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Molecular Methods for Evolutionary Genetics 1st Edition Emily E. Hare Digital Instant Download

Author(s): Emily E. Hare, J. Spencer Johnston (auth.), Virginie Orgogozo, Matthew V. Rockman (eds.)
ISBN(s): 9781617792281, 1617792284
Edition: 1
File Details: PDF, 10.59 MB
Year: 2011
Language: english
SKU: EB-11849814 Category: Tags: , , ,

Molecular Methods for Evolutionary Genetics 1st Edition by Virginie Orgogozo, Matthew Rockman – Ebook PDF Instant Download/Delivery: 1617792284, 9781617792281

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Product details:

ISBN 10: 1617792284 

ISBN 13: 9781617792281

Author: Virginie Orgogozo, Matthew V. Rockman

We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.

Table of contents:

Characterizing the Genome

Genome Size Determination Using Flow Cytometry of Propidium Iodide-stained Nuclei
Emily E. Hare and J. Spencer Johnston

Chromosome Analysis in Invertebrates and Vertebrates
David M. Rowell, Shu Ly Lim, and Frank Grutzner

Genomic Libraries: I. Construction and Screening of Fosmid Genomic Libraries
Mike A. Quail … [et al.]

Genomic Libraries: II. Subcloning, Sequencing, and Assembling Large-insert Genomic DNA Clones
Mike A. Quail … [et al.]

Targeting Regions of the Genome

Reduced Representation Methods for Subgenomic Enrichment and Next-generation Sequencing
Jeffrey M. Good

Accessing the Transcriptome: How to Normalize mRNA Pools
Heiko Vogel and Christopher W. Wheat

Transcriptome Sequencing Goals, Assembly, and Assessment
Christopher W. Wheat and Heiko Vogel

Rapid Retrieval of DNA Target Sequences by Primer Extension Capture
Adrian W. Briggs

Measuring Genetic Diversity

SNP Discovery and Genotyping for Evolutionary Genetics Using RAD Sequencing
Paul D. Etter … [et al.]

DNA Microarray-based Mutation Discovery and Genotyping
David Gresham

Genotyping with Sequenom
Martina Bradić, João Costa, and Ivo M. Chelo

Isolating Microsatellite Loci: Looking Back, Looking Ahead
José A. Andrés and Steven M. Bogdanowicz

Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids Using Array Oligo Selector
Amy A. Caudy

Obtaining Candidate Gene Sequences

Identification of Homologous Gene Sequences by PCR with Degenerate Primers
Michael Lang and Virginie Orgogozo

Characterizing cDNA Ends by Circular RACE
Patrick T. McGrath

Identification of DNA Sequences That Flank a Known Region by Inverse PCR
Anastasios Pavlopoulos

Analyzing Candidate Gene Transcripts

Quantification of Transcript Levels with Quantitative RT-PCR
Karen L. Carleton

Using Pyrosequencing to Measure Allele-specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences
Patricia J. Wittkopp

Whole-mount In Situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis-regulatory Changes in Gene Expression Pattern
Ryo Futahashi

Identifying Fluorescently Labeled Single Molecules in Image Stacks Using Machine Learning
Scott A. Rifkin

Testing Candidate Genes and Candidate Mutations

Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution
Mark Rebeiz and Thomas M. Williams

Experimental Approaches to Evaluate the Contributions of Candidate Protein-coding Mutations to Phenotypic Evolution
Jay F. Storz and Anthony J. Zera

Making Reporter Gene Constructs to Analyze Cis-regulatory Elements
José Bessa and José Luis Gómez-Skarmeta

PCR-directed In Vivo Plasmid Construction Using Homologous Recombination in Baker’s Yeast
Erik C. Andersen

Production of Fosmid Genomic Libraries Optimized for Liquid Culture Recombineering and Cross-species Transgenesis
Radoslaw Kamil Ejsmont … [et al.]

Recombination-mediated Genetic Engineering of Large Genomic DNA Transgenes
Radoslaw Kamil Ejsmont … [et al.]

Overlap Extension PCR: An Efficient Method for Transgene Construction
Matthew D. Nelson and David H.A. Fitch

Gene Knockdown Analysis by Double-stranded RNA Injection
Benjamin N. Philip and Yoshinori Tomoyasu

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Tags: Virginie Orgogozo, Matthew Rockman, Molecular, Methods