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Helicobacter Species Methods and Protocols 1st Edition by JeanMarie Houghton 9781627030052 1627030050

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Helicobacter Species Methods and Protocols 1st Edition Jean Marie Houghton (Auth.) Digital Instant Download

Author(s): Jean Marie Houghton (auth.), JeanMarie Houghton (eds.)
ISBN(s): 9781627030052, 1627030050
Edition: 1
File Details: PDF, 2.92 MB
Year: 2012
Language: english
SKU: EB-4289676 Category: Tags: ,

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Product details:

ISBN 10: 1627030050

ISBN 13: 9781627030052

Author: JeanMarie Houghton

Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states. Over the last several decades, scientists have come to understand that the interplay between the bacteria, the host, and the environment all contribute to the clinical outcome of infection. In Helicobacter Species: Methods and Protocols, expert researchers in the field detail many of the methods and which are now commonly used to study Helicobacter infection. These include protocols and methods that have evolved over time, and standards across the field have been established which are essential for optimal outcomes and to allow comparison of data across different laboratories. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.

Table of contents:

  1. Introduction

  2. Helicobacter pylori: an overview

  3. Perspectives on methodology for in vitro culture of Helicobacter pylori

  4. Successful culture techniques for Helicobacter species: general culture techniques for Helicobacter pylori

  5. Successful culture techniques for Helicobacter species: establishing H. pylori cultures from infected rodents

  6. Successful culture techniques for Helicobacter species: verification of Helicobacter identity using 16s rRNA gene sequence analysis

  7. The Helicobacter pylori cag pathogenicity island

  8. Genetic manipulation of a naturally competent bacterium, Helicobacter pylori

  9. A method for short-term culture of human gastric epithelial cells to study the effects of Helicobacter pylori

  10. Cell culture-based assays to test for bacterial adherence and internalization

  11. Cell culture assays to evaluate bacterial toxicity and virulence

  12. Rodent models of Helicobacter infection, inflammation and disease

  13. Bacterial culture and inoculation of mice (simple infection)

  14. Adoptive transfer of splenocytes to immunocompromised mice

  15. Isolation of gastric lamina propria leukocytes

  16. Delayed-type hypersensitivity determination

  17. Necropsy, blood, tissue collection and mRNA isolation for detection of host cytokine gene expression

  18. Animal models of Helicobacter-induced disease: methods to successfully infect the mouse

  19. Verifying and quantifying Helicobacter pylori infection status of research mice

  20. Mouse models of Helicobacter-induced gastric cancer: use of cocarcinogens

  21. Gastric Helicobacter spp. in animal models: pathogenesis and modulation by extragastric coinfections

  22. Histologic scoring of gastritis and gastric cancer in mouse models

  23. Innate immune responses to Helicobacter pylori infection: an overview

  24. Methods for in vivo and in vitro analysis of innate immune responses to Helicobacter pylori infection

  25. Techniques for following labeled cells in vivo: use of X/Y FISH, techniques to optimize fluorescent detection, and beta-galactosidase detection

  26. In vivo measurement of Helicobacter pylori infection

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Tags: JeanMarie Houghton, Helicobacter, Species, Protocols