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Pyrosequencing Methods and Protocols 2nd Edition by Ulrich Lehmann, Jörg Tost 9781493927142 1493927140

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Pyrosequencing Methods and Protocols 2nd ed. 2015 Edition Ulrich Lehmann Digital Instant Download

Author(s): Ulrich Lehmann, Jörg Tost
ISBN(s): 9781493927142, 1493927140
Edition: 2nd ed. 2015
File Details: PDF, 12.57 MB
Year: 2015
Language: english
SKU: EB-5109202 Category: Tags: ,

Pyrosequencing Methods and Protocols 2nd Edition by Ulrich Lehmann, Jörg Tost – Ebook PDF Instant Download/Delivery: 9781493927142, 1493927140

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Product details:

ISBN 10: 1493927140

ISBN 13: 9781493927142

Author: Ulrich Lehmann, Jörg Tost

The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology.

Table of contents:

  1. The History of Pyrosequencing®

  2. PyroMark® Instruments, Chemistry, and Software for Pyrosequencing® Analysis

  3. Software-Based Pyrogram® Evaluation

  4. Quantitative Validation and Quality Control of Pyrosequencing® Assays

  5. Extended KRAS and NRAS Mutation Profiling by Pyrosequencing®

  6. Universal BRAF State Detection by the Pyrosequencing®-Based U-BRAFV600 Assay

  7. Pyrosequencing®-Based Identification of Low-Frequency Mutations Enriched Through Enhanced-ice-COLD-PCR

  8. Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing®

  9. Analysis of Copy Number Variation by Pyrosequencing® Using Paralogous Sequences

  10. Prenatal Diagnosis of Chromosomal Aneuploidies by Quantitative Pyrosequencing®

  11. HLA-B and HLA-C Supratyping by Pyrosequencing®

  12. Allele Quantification Pyrosequencing® at Designated SNP Sites to Detect Allelic Expression Imbalance and Loss-of-Heterozygosity

  13. Quantitative DNA Methylation Analysis by Pyrosequencing®

  14. Quantitative Methylation Analysis of the PCDHB Gene Cluster

  15. Assessment of Changes in Global DNA Methylation Levels by Pyrosequencing® of Repetitive Elements

  16. Global Analysis of DNA 5-Methylcytosine Using the Luminometric Methylation Assay, LUMA

  17. Limiting Dilution Bisulfite Pyrosequencing®

  18. Detection of Loss of Imprinting by Pyrosequencing®

  19. Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing®

  20. Allele-Specific DNA Methylation Detection by Pyrosequencing®

  21. SNP-Based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing®

  22. DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution by Pyrosequencing®

  23. Multiplex Pyrosequencing®

  24. Detection of Drug-Resistant Mycobacterium tuberculosis

  25. Application of Pyrosequencing® in Food Biodefense

  26. Forensic Analysis of Mitochondrial and Autosomal Markers Using Pyrosequencing®

  27. Tissue-Specific DNA Methylation Patterns in Forensic Samples Detected by Pyrosequencing®

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Tags: Ulrich Lehmann, Jörg Tost, Pyrosequencin, Protocols