Human Papillomaviruses Methods and Protocols Methods in Molecular Biology 1st Edition by Clare Davy, John Doorbar 9781588293732 1588293734
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ISBN 10: 1588293734
ISBN 13: 9781588293732
Author: Clare Davy, John Doorbar
Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The prools follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Table of contents:
Chapter 1: Identification of New Papillomavirus Types
Chapter 2: Identification of HPV Variants
Chapter 3: Histochemical Analysis of Cutaneous HPV-Associated Lesions
Chapter 4: Histological Analysis of Cervical Intraepithelial Neoplasia
Chapter 5: Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections
Chapter 6: Detection and Quantitation of HPV Gene Expression Using Real-Time PCR
Chapter 7: Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers
Chapter 8: Use of Biomarkers in the Evaluation of CIN Grade and Progression of Early CIN
Chapter 9: HPV DNA Detection and Typing in Cervical Scrapes
Chapter 10: HPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant Lesions
Chapter 11: Establishing HPV-Containing Keratinocyte Cell Lines from Tissue Biopsies
Chapter 12: Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic “Raft” Cultures
Chapter 13: Differentiation of HPV-Containing Cells Using Organotypic “Raft” Culture or Methylcellulose
Chapter 14: Propagation of Infectious, High-Risk HPV in Organotypic “Raft” Culture
Chapter 15: Retrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic “Raft” Cultures
Chapter 16: The HPV Xenograft Severe Combined Immunodeficiency Mouse Model
Chapter 17: The Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced Disease
Chapter 18: Studying the HPV Life Cycle in 3A Trophoblasts and Resulting Pathophysiology
Chapter 19: Replication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiae
Chapter 20: Analysis of the Regulation of Viral Transcription
Chapter 21: Analysis of HPV Transcription by RPA
Chapter 22: Analysis of Regulatory Motifs Within HPV Transcripts
Chapter 23: Detection of HPV Transcripts by Nested RT-PCR
Chapter 24: Analysis of HPV DNA Replication Using Transient Transfection and Cell-Free Assays
Chapter 25: Detection and Quantitation of HPV DNA Replication by Southern Blotting and Real-Time PCR
Chapter 26: Analysis of E7/Rb Associations
Chapter 27: Transformation Assays for HPV Oncoproteins
Chapter 28: Analysis of Adeno-Associated Virus and HPV Interaction
Chapter 29: In Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 Oncoproteins
Chapter 30: Measuring the Induction or Inhibition of Apoptosis by HPV Proteins
Chapter 31: Codon Optimization of Papillomavirus Genes
Chapter 32: Generation of HPV Pseudovirions Using Transfection and Their Use in Neutralization Assays
Chapter 33: Generation and Applications of HPV Pseudovirions Using Vaccinia Virus
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Tags: Clare Davy, John Doorbar, Human, Papillomaviruses