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DNA Topoisomerases Methods and Protocols Methods in Molecular Biology Vol 582 1st Edition by Duncan Clarke 1607613395 9781607613398

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DNA Topoisomerases Methods and Protocols Methods in Molecular Biology 582 1st Edition Duncan J. Clarke Digital Instant Download

Author(s): Duncan J. Clarke, Andrew Lane, Duncan J. Clarke
ISBN(s): 9781607613398, 1607613395
Edition: 1
File Details: PDF, 5.00 MB
Year: 2009
Language: english
SKU: EB-1315738 Category: Tags: ,

DNA Topoisomerases Methods and Protocols Methods in Molecular Biology Vol 582 1st Edition by Duncan Clarke – Ebook PDF Instant Download/Delivery: 1607613395, 9781607613398

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Product details:

ISBN 10: 1607613395

ISBN 13: 9781607613398

Author: Duncan J. Clarke

Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In DNA Topoisomerases: Methods and Protocols, experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls.

Cutting-edge and easy-to-use, DNA Topoisomerases: Methods and Protocols provides key content which reflects an expanded understanding of the functions of these essential enzymes in the hope of inspiring researchers to further important discoveries in this dynamic field.

Table of contents:

Emerging themes in DNA topoisomerase research / Duncan J. Clarke and Andrew Lane

Analysis of DNA topoisomers, knots, and catenanes by agarose gel electrophoresis / Stephen D. Levene

Two-dimensional ararose gel electrophoresis of DNA topoisomers / Joaquim Roca

Cleavage of plasmid DNA by eukaryotic topoisomerase II / Omari J. Bandele, Neil Osheroff

Assays for the preferential binding of human topoisomerase I to supercoiled DNA / Zheng Yang and James J. Champoux

Monitoring the topoisomerase II DNA gate conformational change with fluorescence resonance energy transfer / Tammy R.L. Collins and Tao-shih Hsieh

Single-molecule magnetic tweezers studies of type IB topoisomerases / Jan Lipfert, Daniel A. Koster, Igor D. Vilfan, Susanne Hage and Nynke H. Dekker

Dissolution of double holliday junctions by the concerted action of BLM and topoisomerase III / Csanad Z. Bachrati and Ian D. Hickson

ChIP-on-Chip analysis of DNA topoisomerases / Rodrigo Bermejo, Yuki-Mori Katou, Katsuhiko Shirahige and Marco Foiani

Analyzing Top2 distribution on yeast chromosomes by chromatin immunoprecipitation / Melissa Baldwin, Tariq Warsi and Jeff Bachant

Binding of DNA topoisomerases I and II to replication origins / Arturo Falaschi

Cytometric assessment of DNA damage induced by DNA topoisomerase inhibitors / Zbigniew Darzynikiewicz, Dorota H. Halicka and Toshiki Tanaka

Analysis of the topoisomerase II-dependent decatenation G2 checkpoint and checkpoint kinases in human cells / William K. Kaufmann

Assaying topoisomerase II checkpoints in yeast / Katherine Furniss, Amit C.J. Vas, Anderw Lane and Duncan J. Clarke

Cytological analysis of chromosome structural defects that result from topoisomerase II dysfunction / Juan F. Giménez-Abián and Duncan J. Clarke

Top2 SUMO conjugation in yeast cell lysates / Melissa Baldwin and Jeff Bachant

Analysis of SUMOylation of topoisomerase IIα with Xenopus egg extracts / Yoshiaki Azuma

Dynamics of DNA topoisomerase IIα in living cells / John R. Daum, Yin Yuan Mo and Gary J. Gorbsky

Depletion and mutation of topoisomerase II in animal cells / Andrew C.G. Porter

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Tags: Duncan Clarke, Topoisomerases, DNA, Protocols