Mammalian Artificial Chromosomes Methods and Protocols 1st Edition by Vittorio Sgaramella, Sandro Eridani ISBN 1489939487 978-1489939487

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ISBN 10:  1489939487 

ISBN 13: 978-1489939487

Author: Vittorio Sgaramella, Sandro Eridani
In 1996, we organized a workshop, inter alia, at the National Research Co- cil in Milan under the generous sponsorship of the European Science Foun- tion. On that occasion, a small group of investigators convened from many countries and presented early evidence of the possibility of assembling basic units of mammalian chromosomes into artificial constructs (or, indeed, red- ing the relevant components to more manageable dimensions and defined c- stitution). Progress in the following years has been slow but steady. Many scientists who took part in the workshop have since been engaged in active and prod- tive research. It goes to the credit of Humana Press to have realized the need for a book on artificial chromosomes that aims to provide better tools to all scientists committed to this field who are confronted with very difficult tech- cal problems. We have strived to cover in Mammalian Artificial Chromosomes: Methods and Protocols all relevant areas of artificial chromosome research, from basic genetics to daring attempts to build new tools for genetic therapy. We are of course grateful to the authors who have accepted the task of describing the technical steps and pitfalls that can be encountered in their research. Rarely has a very delicate methodology been presented with such meticulous care. We have been helped in this enterprise by the excellent librarian of the LITA Institute in Segrate, Italy, Ms. Claudia Piergigli, whom we thank warmly. Ms.

Table of contents: 

1. From Natural to Artificial Chromosomes
   Vittorio Sgaramella, Sandro Eridani

2. Methodologies Applied to Domestic Animal Chromosomes
   Leopoldo Iannuzzi

3. Mapping of the Hotspots of Recombination in Human DNA Cloned as Yeast Artificial Chromosomes
   Grzegorz Ira, Jacek Król, Jan Filipski

4. Genomic Imprinting and Its Effects on Genes and Chromosomes in Mammals
   Yuji Goto, Robert Feil

5. Centromeres and Neocentromeres
   Gérard Roizés, Christoph Grunau, Jérôme Buard, Albertina De Sario, Jacques Puechberty

6. Identification of Start Sites of Bi-Directional DNA Synthesis at Eukaryotic DNA Replication Origins
   Alessandro Vindigni, Gulnara Abdurashidova, Arturo Falaschi

7. Telomere Length Analysis and In Vitro Telomerase Assay
   Fiorentina Ascenzioni, Pier Assunta Fradiani, Pierluigi Donini

8. Telomerization of Mammalian Cells and Transplantation of Telomerized Cells in Immunodeficient Mice
   Peter J. Hornsby

9. Chromosome-Based Vectors for Mammalian Cells
   Han N. Lim, Christine J. Farr

10. Mammalian Artificial Chromosome Formation in Human Cells After Lipofection of a PAC Precursor
    Jose I. de las Heras, Leonardo D’Aiuto, Howard Cooke

11. Use of Natural and Artificial Chromosome Vectors for Animal Transgenesis
    Yoshimi Kuroiwa, Kazuma Tomizuka, Isao Ishida

12. Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice
    Diane P. Monteith, Josephine D. Leung, Anita H. Borowski, Deborah O. Co, Tünde Praznovszky, Frank R. Jirik et al.

13. Modification of Human Bacterial Artificial Chromosome Clones for Functional Studies and Therapeutic Applications
    Michael R. Orford, Duangporn Jamsai, Samuel McLenachan, Panayiotis A. Ioannou

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Tags: Vittorio Sgaramella, Sandro Eridani, Mammalian Artificial, Chromosomes Methods