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Neutrophil Methods and Protocols 1st Edition by Mark Quinn, Frank DeLeo 1627038442 9781627038447

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Neutrophil Methods and Protocols 1st Edition Mark T. Quinn Digital Instant Download

Author(s): Mark T. Quinn, Frank R. DeLeo (eds.)
ISBN(s): 9781627038447, 1627038442
Edition: 1
File Details: PDF, 6.95 MB
Year: 2014
Language: english
SKU: EB-4658944 Category: Tags: ,

Neutrophil Methods and Protocols 1st Edition by Mark Quinn, Frank DeLeo – Ebook PDF Instant Download/Delivery: 1627038442, 9781627038447

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Product details:

ISBN 10: 1627038442

ISBN 13: 9781627038447

Author: Mark T. Quinn, Frank R. DeLeo

Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity and extracellular trap formation and protocols for investigating neutrophil adhesion, chemotaxis and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Table of contents:

The Role of Neutrophils in the Immune System: An Overview

Isolation of Human Neutrophils

Neutrophil Isolation from Non-Human Species

Collection of In vivo Transmigrated Neutrophils from Human Skin

Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers

Rho Family and Rap GTPase Activation Assays

Measurement of Phospholipid Metabolism in Intact Neutrophils

Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium Signals in Neutrophils

Analysis of Electrophysiological Properties and Responses of Neutrophils

Analysis of Neutrophil Apoptosis

Microinjection Methods for Neutrophils

Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells

Neutrophil Migration Through Extracellular Matrix

Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish

Detection of Bi-Directional Signaling During Integrin Activation and In Neutrophil Adhesion

Immunofluorescence and Confocal Microscopy of Neutrophils

Expression of Genetically-Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils

Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry

Analysis of Neutrophil Bactericidal Activity

Induction and Quantification of Neutrophil Extracellular Traps

Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes

Cell-free NADPH Oxidase Assays: “In vitro Veritas”

Assessment of Priming of the Human Neutrophil Respiratory Burst

Affinity Purification and Reconstitution of Human Phagocyte Flavocytochrome b for Detection of Conformational Dynamics in the Membrane

Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies

Genome-Scale Transcript Analyses with Human Neutrophils

Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils

High Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis

Detection of Intact Transcription Factors in Human Neutrophils

Disorders of Neutrophil Function: An Overview

Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders

Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency

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Tags: Mark Quinn, Frank DeLeo, Neutrophil, Methods