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Human Retrovirus Protocols Virology and Molecular Biology 1st Edition by Tuofu Zhu 1588294951 9781588294951

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Human Retrovirus Protocols Virology and Molecular Biology 1st Edition Janet D. Siliciano Digital Instant Download

Author(s): Janet D. Siliciano, Robert F. Siliciano (auth.), Tuofu Zhu MD (eds.)
ISBN(s): 9781588294951, 1588294951
Edition: 1
File Details: PDF, 9.78 MB
Year: 2005
Language: english
SKU: EB-1315214 Category: Tags: , ,

Human Retrovirus Protocols Virology and Molecular Biology 1st Edition by Tuofu Zhu – Ebook PDF Instant Download/Delivery: 1588294951, 9781588294951

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ISBN 10: 1588294951 

ISBN 13: 9781588294951

Author: Tuofu Zhu

A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.

Table of contents:

  1. Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals

  2. Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals

  3. Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes

  4. Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid

  5. Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue

  6. Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids

  7. Isolation and Quantification of HIV From Lymph Nodes

  8. Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes

  9. Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells

  10. Isolation of Foamy Viruses From Peripheral Blood Lymphocytes

  11. Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA

  12. Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR

  13. Detection of HIV-1 Provirus and RNA by In Situ Amplification

  14. Detection of HTLV-1 Gene on Cytologic Smear Slides

  15. Detection of HIV-2 by PCR

  16. Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions

  17. Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR

  18. Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR

  19. A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay

  20. A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity

  21. Detection of Antiviral Antibodies Using Enzyme-Linked Immunosorbent Assay

  22. Plaque-Reduction Assays for Human and Simian Immunodeficiency Virus Neutralization

  23. Detection of Drug-Resistant HIV-1 Strains

Molecular Biology Methods

  1. Determination of Cell Tropism of HIV-1

  2. Determination of Co-Receptor Usage of HIV-1

  3. Quantitative Evaluation of HIV and SIV Co-Receptor Use With GHOST(3) Cell Assay

  4. Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals

  5. Methods to Determine HIV-1 Ex Vivo Fitness

  6. A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes

  7. PCR Amplification, Cloning, and Construction of HIV-1 Infectious Molecular Clones From Virtually Full-Length HIV-1 Genomes

  8. Amplification and Cloning of Near Full-Length HIV-2 Genomes

  9. Growth and Manipulation of a Human T-Cell Leukemia Virus Type 2 Full-Length Molecular Clone

  10. Construction and Analysis of Genomic, Full-Length Infectious Foamy Virus DNA Clones

  11. Molecular Characterization of Proteolytic Processing of the Gag Proteins of Human Spumaretrovirus

  12. Assessing the Relative Efficacy of Antiretroviral Activity of Different Drugs on Macrophages

  13. Gene Expression Profiling of HIV-1 Infection Using cDNA Microarrays

  14. Determination of DC-SIGN and DC-SIGNR Repeat Region Variations

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Tags: Tuofu Zhu, Retrovirus, Protocols, Virology