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Alcohol Methods and Protocols Methods in Molecular Biology 1st Edition by Laura Nagy 1588299066 9782007938043

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Alcohol Methods and Protocols Methods in Molecular Biology 1st Edition Laura E. Nagy Digital Instant Download

Author(s): Laura E. Nagy
ISBN(s): 9782007938043, 1588299066
Edition: 1
File Details: PDF, 4.82 MB
Year: 2008
Language: english
SKU: EB-1311710 Category: Tag:

Alcohol Methods and Protocols Methods in Molecular Biology 1st Edition by Laura Nagy – Ebook PDF Instant Download/Delivery: 1588299066, 9782007938043

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Product details:

ISBN 10: 1588299066 

ISBN 13: 9782007938043

Author: Laura E. Nagy

Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.

Table of contents:

  1. Animal Models of Acute and Chronic Ethanol Exposure

  2. Acute Models of Ethanol Exposure to Mice

  3. A Voluntary Oral-feeding Rat Model for Pathological Alcoholic Liver Injury

  4. Intragastric Ethanol Infusion Model in Rodents

  5. A Practical Method of Chronic Ethanol Administration in Mice

  6. Animal Models of Ethanol Exposure During Development

  7. Analysis of Ethanol Developmental Toxicity in Zebrafish

  8. The Avian Embryo in Fetal Alcohol Research

  9. Artificial Rearing

  10. Intragastric Intubation of Alcohol During the Perinatal Period

  11. Cell Culture Approaches to Studying Ethanol Exposure

  12. Human Monocytes, Macrophages, and Dendritic Cells: Alcohol Treatment Methods

  13. Generation and Use of Primary Rat Cultures for Studies of the Effects of Ethanol

  14. Development and Properties of HepG2 Cells That Constitutively Express CYP2E1

  15. Modeling the Impact of Alcohol on Cortical Development in a Dish: Strategies from Mapping Neural Stem Cell Fate

  16. Regulation of Specific Organ Systems in Response to Ethanol

  17. Acetaldehyde-induced Barrier Disruption and Paracellular Permeability in Caco-2 Cell Monolayer

  18. Alcohol-Induced Oxidative Stress in the Liver: In Vivo Measurements

  19. Isolation of Kupffer Cells from Rats Fed Chronic Ethanol

  20. Dendritic Cells in Chronic In Vivo Ethanol Exposure Models

  21. Formation and Immunological Properties of Aldehyde-derived Protein Adducts following Alcohol Consumption

  22. Assessment of Natural Killer (NK) and NKT Cells in Murine Spleens and Livers

  23. Polyclonal and Antigen-specific Responses of T Cells and T Cell Subsets

  24. B-Cell Studies in Chronic Ethanol Mice

  25. Histological Analysis of Bone

  26. Assessing Effects of Alcohol Consumption on Protein Synthesis in Striated Muscles

  27. Methods to Investigate the Effects of Chronic Ethanol on Adipocytes

  28. Proteomic and Genomic Approaches to Study Response to Ethanol

  29. Proteomic Approaches to Identify and Characterize Alterations to the Mitochondrial Proteome in Alcoholic Liver Disease

  30. Alcoholic Liver Disease and the Mitochondrial Ribosome: Methods of Analysis

  31. Microarray Analysis of Ethanol-Induced Changes in Gene Expression

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Tags: Laura Nagy, Alcohol, Method, Protocols, Methods