Sale!

Plant Endosomes Methods and Protocols 1st Edition by Marisa Otegui 1493914197 9781493914197

Original price was: $50.00.Current price is: $35.00.

Plant Endosomes Methods and Protocols 1st Edition Marisa S. Otegui (Eds.) Digital Instant Download

Author(s): Marisa S. Otegui (eds.)
ISBN(s): 9781493914197, 1493914197
Edition: 1
File Details: PDF, 10.30 MB
Year: 2014
Language: english
SKU: EB-4739846 Category: Tag:

Plant Endosomes Methods and Protocols 1st Edition by Marisa Otegui – Ebook PDF Instant Download/Delivery: 1493914197, 9781493914197

Full download Plant Endosomes Methods and Protocols 1st Edition after payment

Product details:

ISBN 10: 1493914197

ISBN 13: 9781493914197

Author: Marisa S. Otegui 

Plant Endosomes: Methods and Protocols explores a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, as well as protein-protein interactions in the endosomal and endomembrane system. The volume continues with coverage of the sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal trafficking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins and the identification of novel endosomal components by chemical genomics and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Detailed and practical, Plant Endosomes: Methods and Protocols gathers contributions from many leading and emerging plant membrane trafficking researchers in order to promote and facilitate novel studies and ideas in this vital field.

Plant Endosomes Methods and Protocols 1st Table of contents:

  1. Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis
  2. Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles
  3. Analysis of Endoplasmic Reticulum–Endosome Association Using Live-Cell Imaging in Plant Cells
  4. Degradation of Abscisic Acid Receptors Through the Endosomal Pathway
  5. Biochemical and Imaging Analysis of ALIX Function in Endosomal Trafficking of Arabidopsis Protein Cargoes
  6. Correlative Light and Electron Microscopy Imaging of the Plant trans-Golgi Network
  7. Imaging Plant Cells by High-Pressure Freezing and Serial Block-Face Scanning Electron Microscopy
  8. Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking
  9. Assessing Extrinsic Membrane Protein Dependency to PI4P Using a Plasma Membrane to Endosome Relocalization Transient Assay in Nicotiana benthamiana
  10. Subcellular Localization of PI3P in Arabidopsis
  11. Immunopurification of Intact Endosomal Compartments for Lipid Analyses in Arabidopsis
  12. Cell-Free Protein Translation System for Expression of Lipid-Binding Proteins Tagged with Small Epitopes and Their Use in Protein–Lipid Overlay Assays
  13. Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants
  14. Detection of Phosphorylation on Immunoprecipitates from Total Protein Extracts of Arabidopsis thaliana Seedlings
  15. Purification and Interaction Analysis of a Plant-Specific RAB5 Effector by In Vitro Pull-Down Assay

People also search for Plant Endosomes Methods and Protocols 1st: 

6 plant hormones

5 plant hormones

1 plant antioch

3-2-1 protocol

Tags:

Marisa Otegui,Plant,Endosomes,Methods