Methods for Analysis of Golgi Complex Function 1st Edition by Franck Perez, David Stephens 9780124171640 0124171648

Methods for Analysis of Golgi Complex Function 1st Edition by Franck Perez, David Stephens – Ebook PDF Instant Download/Delivery:9780124171640, 0124171648
Full download Methods for Analysis of Golgi Complex Function 1st Edition  after payment

Product details:

ISBN 10: 0124171648
ISBN 13: 9780124171640 
Author: Franck Perez, David J. Stephens

This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Methods for Analysis of Golgi Complex Function 1st Table of contents:

Part 1: Model Systems and Functional Studies
Chapter 1. Analysis of Golgi Complex Functions: In Vitro Reconstitution Systems

• Abstract
• Introduction
• Acknowledgments
• References

Chapter 2. RNA Interference Approaches to Examine Golgi Function in Animal Cell Culture

• Abstract
• Overview of Gene Depletion Methodologies
  • 2.1 Key Considerations for the Analysis of Organelle Function
  • 2.2 Strategies for RNAi Experiments
  • 2.3 Choosing Between siRNA and shRNA
  • 2.4 Sources of Reagents for RNAi
  • 2.5 Controls
  • 2.6 Methods
  • 2.7 Discussion
• Summary
• Acknowledgments
• References

Chapter 3. Trafficking Along the Secretory Pathway in Drosophila Cell Line and Tissues: A Light and Electron Microscopy Approach

• Abstract
• Introduction and Rationale
  • 3.1 Materials
  • 3.2 Methods
• Conclusions/Perspectives
• Acknowledgments
• References

Chapter 4. Studying Membrane Trafficking in the Worm C. elegans by RNA Interference

• Abstract
• Introduction and Rationale
  • 4.1 Materials
  • 4.2 Methods
  • 4.3 Discussion
• Summary
• Acknowledgment
• References

Chapter 5. Endomembrane and Golgi Traffic in Plant Cells

• Abstract
• Introduction
  • 5.1 Leaves as a Model System for Analyzing the Plant Golgi and Secretory Pathway
  • 5.2 Mutant Screens for Trafficking Proteins
  • 5.3 Photobleaching for Transport Studies
  • 5.4 Förster Resonance Energy Transfer for Protein Interactions
• Summary
• Acknowledgments
• References

Chapter 6. Biochemical Analysis of Secretory Trafficking in Mammalian Cells

• Abstract
• Introduction
  • 6.1 Materials
  • 6.2 Methods
  • 6.3 Discussion
• Summary
• Acknowledgments
• References

Chapter 7. Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems

• Abstract
• Introduction
  • 7.1 3D Culture of MDCK Cysts on Micropatterns
  • 7.2 Discussion
• Acknowledgments
• References

Chapter 8. Using Replication Defective Viruses to Analyze Membrane Trafficking in Polarized Epithelial Cells

• Abstract
• Introduction and Rationale
  • 8.1 Materials
  • 8.2 Methods
  • 8.3 Discussion
• Summary
• Acknowledgments
• References

Chapter 9. SNAP-Tagging the Retrograde Route

• Abstract
• Introduction
  • 9.1 Materials
  • 9.2 Methods
  • 9.3 Conclusion/Discussion
• Summary
• Acknowledgments
• References

People also search for Methods for Analysis of Golgi Complex Function 1st :

golgi complex function and structure
    
golgi apparatus complex function
    
golgi complex/ apparatus
    
golgi method
    
a golgi apparatus

Tags:

Franck Perez,David Stephens,Methods,Analysis