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Oat Methods and Protocols 1st Edition by Sebastian Gasparis 1493966804 9781493966806

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Oat 1st Edition Sebastian Gasparis Digital Instant Download

Author(s): Sebastian Gasparis
ISBN(s): 9781493966806, 1493966804
Edition: 1st
File Details: PDF, 6.41 MB
Year: 2017
Language: english
SKU: EB-47740630 Category: Tag:

Oat Methods and Protocols 1st Edition by Sebastian Gasparis – Ebook PDF Instant Download/Delivery: 1493966804, 9781493966806

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Product details:

ISBN 10: 1493966804 

ISBN 13: 9781493966806

Author: Sebastian Gasparis

The volume provides detailed protocols that have been developed or modified exclusively for the study of oat. The topics discussed in this book are a selection of various molecular biology and biotechnology methods, such as the application of molecular markers for polymorphism analyses and cytological manipulations, the production of synthetic polyploids, and in vitro cultures and genetic modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Oat: Methods and Protocols is a useful resource in the development of new research approaches toward organizing the oat genome and the identification of new and useful traits for further improvements of this exceptional crop.

Table of contents:

  1. Fluorescence In Situ Hybridization in Oat

  2. Oat Doubled Haploids Following Maize Pollination

  3. Wide Hybridization between Oat and Pearl Millet

  4. Protocol for Producing Synthetic Polyploid Oats

  5. Manipulation of Oat Protoplasts for Transient Expression Assays

  6. Oat Anther Culture and Use of DH-Lines for Genetic Mapping

  7. Agrobacterium-Mediated Transformation of Leaf Base Segments

  8. Chromatographic Methods to Evaluate Nutritional Quality in Oat

  9. Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid Chromatography–Tandem Mass Spectrometry

  10. Multiplex Dipstick Immunoassay for Semi-Quantitative Determination of Fusarium Mycotoxins in Oat

  11. Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat

  12. M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm

  13. Genotyping-By-Sequencing and its Application to Oat Genomic Research

  14. Genome-Wide Association Analysis using R

  15. de novo Transcriptome Assembly in Polyploid Species

  16. Isolation of Oat (Avena sativa L.) Total Proteins and their Prolamin Fractions for 2-D Electrophoresis

  17. 2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and their Prolamin Fractions

  18. Selected Bioinformatic Tools and MS (MALDI-TOF, PMF) Techniques Used in the Strategy for the Identification of Oat Proteins after 2-DE

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Tags: Sebastian Gasparis, Methods, Protocols