Electron Microscopy Methods and Protocols 2nd Edition by John Kuo 1588295737 9781588295736

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ISBN 10: 1588295737

ISBN 13: 9781588295736

Author: John Kuo

This book presents the newest technology in electron microscopy. It comprises two major areas of electron microscopy – transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. A notes section enables efficient adaptation and troubleshooting of protocols.

Electron Microscopy Methods and Protocols 2nd Table of contents:

1. Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells
2. Processing Biological Tissues for Ultrastructural Study
3. Processing Plant Tissues for Ultrastructural Study
4. Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy
5. Ultramicrotomy for Biological Electron Microscopy
6. Poststaining Grids for Transmission Electron Microscopy
7. Negative Staining of Thinly Spread Biological Samples
8. Recent Advances in High-Pressure Freezing
9. Cryoultramicrotomy
10. Cell-Free Extract Systems and the Cytoskeleton
11. Electron Microscopy In Situ Hybridization
12. Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections
13. Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry
14. Fluorescence-Integrated Transmission Electron Microscopy Images
15. Quantitative Immunoelectron Microscopy
16. Electron Crystallography of Membrane Proteins
17. Cryoelectron Microscopy of Icosahedral Virus Particles
18. Three-Dimensional Reconstruction of Chromosomes Using Electron Tomography
19. Whole-Mount Immunoelectron Tomography of Chromosomes and Cells
20. Three-Dimensional Cryotransmission Electron Microscopy of Cells and Organelles
21. Electron Energy-Loss Spectroscopy as a Tool for Elemental Analysis in Biological Specimens
22. Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens
23. Variable Pressure and Environmental Scanning Electron Microscopy
24. Cryoplaning Technique for Visualizing the Distribution of Water in Woody Tissues by Cryoscanning Electron Microscopy
25. X-Ray Microanalysis in the Scanning Electron Microscope
26. Botanical X-Ray Microanalysis in Cryoscanning Electron Microscopy
27. Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research
28. Using SIMS and MIMS in Biological Materials
    

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