A Practical Manual for Musculoskeletal Research 1st Edition by Leung Kwok Sui, Leung Kwok Sui, Ling Qin, Wing hoi Cheung 9812706100 9789812706102

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Product details:

ISBN 10: 9812706100

ISBN 13: 9789812706102

Author: Leung Kwok-Sui, Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung

This manual provides technical protocols for musculoskeletal research on a translational basis, i.e. a disease-orientated approach. It offers guidance on various laboratory techniques, including cell culture and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal models, CT- and MRI-based densitometry and microarchitectural analysis, biomechanics and functional analysis of orthopedic kinesiology, etc. The content is simple and straightforward, with illustrations and step-by-step procedures as an easy experimental reference for personnel in basic and clinical musculoskeletal research and education. This book will provide a unique multidisciplinary platform for various professions — not only orthopedics, but also biomedical engineering and biomaterial sciences — involving both basic and clinical medicine.

Table of contents:

Part I: Cell Culture and Molecular Biology

1. DNA Microarray – Xiao-Ling Zhang and Yan-Zhi Du

2. Visualizing Gene Products: Immunohistochemistry, in situ Hybridization, and Staining

3. Mesenchymal Stem Cell Culture, Expansion, and Osteogenic and Adipogenic Differentiation – Hu

4. Stem Cells and Their Role in Bone Formation and Regeneration – Yi-Zhi Meng and Yi-Xian Qin

5. Stem Cells and Tissue Engineering Applications in the Musculoskeletal System – Chang-Hun Lee

6. Osteoblast Culture and Pharmacological Evaluation in vitro – Hong-Fu Wang, Wei-Fang Jin, Jia

7. Osteoclast Culture and Pharmacological Evaluation in vitro – Jian-Jun Cao, Wei-Fang Jin, Hon

8. Primary Cultures of Human Periosteal Cells – Wing-Hoi Cheung, Wing-Sze Lee, C. Zhang, and Kwo

9. Visualization of Osteocytes and Mineralization – Yi-Xia Xie, Ling Ye, Shu-Bin Zhang, Vladimir

10. Tissue Culture of Giant Cell Tumor of Bone – Lin Huang and Ming-Hao Zheng

11. Chondrocyte Mechanotransduction in Three-Dimensional Cell Culture – Xu Yang, Riaz Gillani

12. Chondrocyte-Pellet Culture for Cartilage Repair Research – Wing-Hoi Cheung, Kwoon-Ho Chow

Part II: Histology and Histomorphometry

13. Tissue Preparations – Yong-Bo Lu, Yi-Xia Xie and Jian-Quan Feng

14. Acceleration of Bone Decalcification by Ultrasound – Xia Guo and Wai-Ling Lam

15. Stains of Bone and Cartilage – Yong-Bo Lu, Yi-Xia Xie and Jian-Quan Feng

16. Contact Microradiography for Studying the Degree of Bone Mineralization – Yong-Ping Cao, Ta

17. Undecalcified Histology in Studying Hard Tissue Implanted with Calcium Phosphate–based Materials

Part III: Microscopy and Bioimaging

18. Protocols of Micro-Computed Tomographic Analysis Established for Musculoskeletal Applications

19. Microangiography for Studying Neovascularization During Long Bone Fracture Repair in a Rat Model

20. High-Resolution Imaging of Organs and Tissues by in vivo Micro-Computed Tomography – Engin

21. Positron Emission Tomography of Bone in Small Animals – Erik Mittra, Shahriar S. Yaghoubi

Part IV: Laboratory Animal Models

22. Surgical Anesthesia and Analgesia for Animals in Musculoskeletal Research – Dewi K. Rowland

23. Mouse Model of Calvarial Osteolysis – Chao Zhang and Ting-Ting Tang

24. Distraction Osteogenesis Model – Chun-Wai Chan and Kwok-Sui Leung

25. Fracture Nonunion Animal Model – Xia Guo, Mu-Qing Liu, Chi-Cheung Hui and Zheng Guo

26. Establishment of Osteoporosis Model in Goats – Wing-Sum Siu, Ling Qin and Kwok-Sui Leung

27. Posterior Spinal Fusion Model – Chun-Wai Chan and Jack Chun-Yiu Cheng

28. Functional Disuse Model for Musculoskeletal Adaptation – Ho-Yan Lam and Yi-Xian Qin

29. Neurogenic Limb Disuse Animal Models – Xia Guo, Xiao-Yun Wang and Wai-Ling Lam

30. Establishment of Steroid-Associated Osteonecrosis Rabbit Model – Ge Zhang, Ling Qin and Hui

31. Establishment of Anterior Cruciate Ligament Reconstruction Model in Rabbit – Chun-Yi Wen

32: Establishment of Normal and Delayed Bone–Tendon Junction Repair Models

33: Anterior Cruciate Ligament Transection (ACLT)-Induced Osteoarthritis in Rats

34: Establishment of Rabbit Partial Growth Plate Defect Model

35: Micro-CT 3D Image Analysis Techniques for Orthopedic Applications: Metal Implant-to-Bone

36: Application of DXA to Assess Orthopedic Implants

37: Clinical Monitoring of Bone Mineralization in Distraction Osteogenesis Using DXA

38: In vivo and ex vivo Bone Mineral Density and Structure Measurements Using XtremeCT

39: Advanced 3D Image Processing Methods for Quantifying Proximal Femur and Vertebra Structure

40: Micro-Finite Element Analysis of Bone

41: The Characterization of Cortical Bone Water Distribution and Structure Changes on Age, Microdamage, and Disuse

42: Dynamic Contrast-Enhanced Magnetic Resonance Imaging of the Musculoskeletal System: Basic Principles and Clinical Applications

43: Noninvasive Evaluation of Knee Cartilage Morphology by Magnetic Resonance Imaging

44: Cell Traction Force Microscopy for Musculoskeletal Research

45: Nanoindentation: Techniques and Technical Considerations for Musculoskeletal Research

46: Micromechanical Testing of Bone Tissues in Tension

47: Technical Manual for Biomechanical Testing of Musculoskeletal Tissues

48: Motion Analysis in Musculoskeletal Research

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