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Calcium Binding Protein Protocols Volume 1 Reviews and Case Studies 1st Edition by Hans Vogel 9780896036888 089603688X

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Calcium Binding Protein Protocols Volume 1 Reviews and Case Studies 1st Edition Hans J. Vogel Digital Instant Download

Author(s): Hans J. Vogel, Richard D. Brokx, Hui Ouyang (auth.), Hans J. Vogel (eds.)
ISBN(s): 9780896036888, 089603688X
Edition: 1
File Details: PDF, 4.02 MB
Year: 2002
Language: english
SKU: EB-932160 Category: Tags: , , ,

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Product details:

ISBN 10: 089603688X

ISBN 13: 9780896036888

Author: Hans J. Vogel

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Table of contents:

  1. Introduction and Reviews

  2. Calcium-Binding Proteins

  3. Calcium

  4. Crystal Structure of Calpain and Insights into Ca²⁺-Dependent Activation

  5. The Multifunctional S100 Protein Family

  6. Ca²⁺ Binding to Proteins Containing γ-Carboxyglutamic Acid Residues

  7. The Caseins of Milk as Calcium-Binding Proteins

  8. Preparation of Recombinant Plant Calmodulin Isoforms

  9. Isolation of Recombinant Cardiac Troponin C

  10. Skeletal Muscle Troponin C

  11. Purification of Recombinant Calbindin D9k

  12. S100 Proteins

  13. Cadherins

  14. α-Lactalbumin and (Calcium-Binding) Lysozyme

  15. Recombinant Annexin II Tetramer

  16. Purification and Characterization of ALG-2: A Novel Apoptosis-Linked Ca²⁺-Binding Protein

  17. Crystallization and Structural Details of Ca²⁺-Induced Conformational Changes in the EF-Hand Domain VI of Calpain

  18. Neurocalcin

  19. Crystallization and Structure-Function of Calsequestrin

  20. Use of Fluorescence Resonance Energy Transfer to Monitor Ca²⁺-Triggered Membrane Docking of C2 Domains

  21. Ca²⁺-Binding Mode of the C2A-Domain of Synaptotagmin

  22. Study of Calcineurin Structure by Limited Proteolysis

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Tags: Hans Vogel, Calcium, Binding, Protein, Protocols