Cancer Stem Cells Methods and Protocols 1st Edition by John Yu 1588299384 9781588299383

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ISBN 10: 1588299384

ISBN 13: 9781588299383

Author: John S. Yu

Cancer is a devastating disease that affects millions of people in the world. Our the- pies for tumors have mostly been based on classical chemotherapy and antiproliferative treatments. More recently, directed therapies against a causative oncogene have led to prominent reduction in tumor rates for some cancers. For instance, chronic myelogenous leukemia that is due to a BCR–ABL translocation can be targeted with Gleevec. Despite these advances in individual tumors, a number of patients are treated for their primary tumors and ultimately relapse. Relapsing tumors can be due to resistance to chemotherapy or to antioncogene drugs. Another hypothesis is that heterogeneity in the tumor leads to an inability of classical chemotherapy to completely eradicate all cells of the tumor. This concept of heterogeneity has led some investigators to propose a cancer stem cell model. As patients are treated with chemotherapy, most of the dividing cells are killed, but this leaves a small subset of cells that have the ability to remake the entire tumor. These are cancer stem cells. They possess the signals of self-renewal and yet can also differentiate. If one could understand more about the cells that remain after classical chemotherapy or the cells that can remake the tumor among the heterogeneous population, this information would have a huge impact on our treatment of cancer.

Cancer Stem Cells Methods and Protocols 1st Table of contents:

1 Defining Cancer Stem Cells by Xenotransplantation in Zebrafish Michael C. Dovey and Leonard I. Zon

2 Analysis of Cancer Stem Cell Metastasis in Xenograft Animal Models. Yibin Kang

3 Identification of Murine and Human Acute Myeloid Leukemia Stem Cells. Aniruddha J. Deshpande, Farid Ahmed, and Christian Buske

4 Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal. Ichiro Nakano and Harley I. Kornblum

5 Prospective Identification of Cancer Stem Cells with the Surface Antigen CD133. Kyeung Min Joo and Do-Hyun Nam

6 Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem Cells. Hugo Guerrero-Cázares, Kaisorn L. Chaichana, and Alfredo Quiñones-Hinojosa

7 Methodologies in Assaying Prostate Cancer Stem Cells.. Hangwen Li, Ming Jiang, Sofia Honorio, Lubna Patrawala, Collene R. Jeter, Tammy Calhoun-Davis, Simon W. Hayward, and Dean G. Tang

8 Characterization of Nonmalignant and Malignant Prostatic Stem/Progenitor Cells by Hoechst Side Population Method Murielle Mimeault and Surinder K. Batra

9 Anchorage-Independent Growth of Prostate Cancer Stem Cells Shunyou Wang

10 Identification of Human Pancreatic Cancer Stem Cells. Chenwei Li, C. J. Lee, and Diane M. Simeone

11 Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma Laurie Ailles and Mark Prince

12 Pituitary Adenoma Stem Cells Patrizia Tunici and John S. Yu

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