Cardiovascular Disease Volume 2 Molecular Medicine Methods in Molecular Medicine 1st Edition by Qing Wang 1617378291 9781617378294

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ISBN 10: 1617378291

ISBN 13: 9781617378294

Author: Qing Wang

Cardiovascular disease is the leading cause of death in developed countries, but is quickly becoming an epidemic in such well-populated countries as China, India, and other developing nations. Cardiovascular research is the key to the prevention, diagnosis, and management of cardiovascular disease. Vigorous and cross-disciplinary approaches are required for successful card- vascular research. As the boundries between different scientific disciplines, particularly in the life sciences, are weakening and disappearing, a successful investigator needs to be competent in many different areas, including genetics, cell biology, biochemistry, physiology, and structural biology. The newly developed field of molecular medicine is a cross-disciplinary science that seeks to comprehend disease causes and mechanisms at the molecular level, and to apply this basic research to the prevention, diagnosis, and treatment of diseases and disorders. This volume in the Methods in Molecular Medicine series, C- diovascular Disease, provides comprehensive coverage of both basic and the most advanced approaches to the study and characterization of cardiovascular disease. These methods will advance knowledge of the mechanisms, diagnoses, and treatments of cardiovascular disease. Cardiovascular Disease is a timely volume in which the theory and pr- ciples of each method are described in the Introduction section, followed by a detailed description of the materials and equipment needed, and step-by-step protocols for successful execution of the method. A notes section provides advice for potential problems, any modifications, and alternative methods.

Cardiovascular Disease Volume 2 Molecular Medicine Methods in Molecular Medicine 1st Table of contents:

1. Microarray Analysis in Cardiovascular Diseases
2. Proteomics Using Two-Dimensional Gel Electrophoresis and Mass Spectrometry in Cardiovascular Research
3. Development and Evaluation of Genomics- and Proteomics-Based Diagnostic Tests
4. Animal Models for Cardiovascular Disease
5. Transgenic Mouse Generation for Cardiovascular Research
6. Quantitative Atherosclerosis Assay in the Mouse Aortic Root
7. Animal Models for Heart Failure Studies
8. Animal Models for Hypertension and Blood Pressure Monitoring
9. Animal Models for Cardiac Arrhythmias
10. Optical Mapping of Shock-Induced Arrhythmias in Rabbit Hearts With Healed Myocardial Infarction
11. Methods for Studying Voltage-Gated Sodium Channels in Heterologous Systems
12. Laser-Induced Thrombosis in Zebrafish Larvae
13. Isolation of Endothelial and Smooth Muscle Cells and Proliferation Assays
14. Adenoviral Vector-Mediated Gene Transfer in Cardiovascular Research
15. Murine and HIV-Based Retroviral Vectors for Gene Transfer Studies
16. Nucleofection® for Efficient Transfection of Primary Cells in Cardiovascular Research
17. Cell Adhesion and Migration Assays
18. Apoptosis Assays
19. Protein Crystal Preparation for X-Ray Structural Studies
20. NMR Spectroscopy for Integrin α IIb β 3 Cytoplasmic Domain Structural Analysis
21. Electron Cryo-Microscopy Applications in Cardiovascular Research
22. Stem Cells in Cardiovascular Disease Research
    

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