Cell Cell Interactions Methods and Protocols 2nd Edition by Troy Baudino 9781627036047 1627036040
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Product details:
ISBN 10: 1627036040
ISBN 13: 9781627036047
Author: Troy A. Baudino
In the second edition of Cell-Cell Interactions: Methods and Protocols, expert researchers in the field detail a collection of protocols to examine interactions between cells. These include protocols that focus on both in vivo and in vitro methods-based approaches. New updated chapters also bring together many currently used assays in examining cell-cell interactions and the biological consequences of those interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, Cell-Cell Interactions: Methods and Protocol, Second Edition is a valuable resource for researchers who are already involved in the cell-cell interaction field, and also for those that are new to the area.
Table of contents:
Chapter 1 Proteomics Analysis of Contact-Initiated Eph Receptor–Ephrin Signaling
Chapter 2 Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Cells and Pericytes
Chapter 3 Analyzing Cell–Cell Interactions in 3-Dimensional Adhesion Assays
Chapter 4 Production of Spontaneously Beating Neonatal Rat Heart Tissue for Calcium and Contractile Studies
Chapter 5 Paracrine Communication Between Mechanically Stretched Myocytes and Fibroblasts
Chapter 6 Assessing Blood–Brain Barrier Function Using In Vitro Assays
Chapter 7 Methods to Assess Tissue Permeability
Chapter 8 In Vivo Quantification of Metastatic Tumor Cell Adhesion in the Pulmonary Microvasculature
Chapter 9 Cell Membrane Vesicles as a Tool for the Study of Direct Epithelial–Stromal Interaction: Lessons from CD147
Chapter 10 Microencapsulation of Stem Cells to Study Cellular Interactions
Chapter 11 Cell-Surface Protein–Protein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies
Chapter 12 Single Cell Analysis of Lipid Rafts
Chapter 13 Micropatterning Cell Adhesion on Polyacrylamide Hydrogels
Chapter 14 Measuring Cell–Cell Tugging Forces Using Bowtie-Patterned mPADs (Microarray Post Detectors)
Chapter 15 Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells
Chapter 16 Proteomic Analysis of the Left Ventricle Post-myocardial Infarction to Identify In Vivo Candidate Matrix Metalloproteinase Substrates
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Tags: Troy Baudino, Interactions, Methods, Protocols