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Cytokines and Colony Stimulating Factors Methods and Protocols 1st Edition by Dieter Körholz, Wieland Kiess 9781588290359 1588290352

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Cytokines and Colony Stimulating Factors Methods and Protocols 1st Edition Dieter Körholz Digital Instant Download

Author(s): Dieter Körholz, Wieland Kiess
ISBN(s): 9781588290359, 1588290352
Edition: 1
File Details: PDF, 5.80 MB
Year: 2002
Language: english
SKU: EB-925048 Category: Tags: ,

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ISBN 10: 1588290352

ISBN 13: 9781588290359

Author: Dieter Körholz, Wieland Kiess

The immune system is a complex network in which different cell types and soluble factors interact to efficiently eliminate various kinds of microorganisms as well as aberrant cell clones. The roots of immunologic investigations reach far into the past. In 430 BC, Thucydides reported that survivors of the plague did not present a second time with similar symptoms. The first report of a successful immu- therapy was made by Edward Jenner in 1798 who found a protective effect of cowpox vaccination against human pox. Since then, much knowledge has been accumulated; today, investigations of the molecular mechanisms of immune regulation are of central research interest. The novel insights into gene polymorphisms and gene regulation gathered from this work has improved our knowledge of individual immune reactions and risk factors in overcoming infections. Strategies to use the immune system for cancer treatment have been propelled by the discovery of divergent immunoregulatory cytokines and the introduction of new gene therapy strategies to modify immune responses. Recently, the discovery of various dendritic cells has focused attention on these cell types as central elements of the immune response and to the possibility of dendritic cell expansion, maturation, and consecutive stimulation with immuno- active tumor-specific peptides. Similarly, methods for ex vivo expansion of various stem cell-derived cell types have led to an improved therapeutic management of various benign and malignant diseases.

Table of contents:

Chapter 1: Primary Immunodeficiencies Caused by Defects of Cytokines and Cytokine Receptors

Chapter 2: Detection Assays for Cytokines and Growth Factors

Chapter 3: Intracellular Detection of T-Cell Cytokines

Chapter 4: Analysis of Cytokine Profiles in Human Skin

Chapter 5: Intracytoplasmic Detection of Proinflammatory Cytokines and Chemokines in Monocytes by Flow Cytometry

Chapter 6: Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells

Chapter 7: Evaluation of the Frequency of Virus-Specific CD8+ T Cells by Cytokine Flow Cytometry

Chapter 8: Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms

Chapter 9: Apoptosis Induction by TRAIL

Chapter 10: Angiogenic Cytokines

Chapter 11: Experimental Strategies for Combined Suicide and Immune Cancer Gene Therapy

Chapter 12: Gene Therapy with Plasmids Encoding Cytokine- or Cytokine Receptor-IgG Chimeric Proteins

Chapter 13: Interferon-γ Gene Therapy for the Treatment of Arthritis

Chapter 14: Gene Gun-Based In Vivo Gene Transfer

Chapter 15: Purification of the Eucaryotic Heat-Shock Proteins Hsp70 and gp96

Chapter 16: Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-α Moiety

Chapter 17: Genetic Engineering of Dendritic Cells by Adenovirus-Mediated TNF-α Gene Transfer

Chapter 18: Retroviral Transfer of T-Cell Receptor Genes into Human Peripheral Blood Lymphocytes

Chapter 19: Gene Transfer to Articular Chondrocytes with Recombinant Adenovirus

Chapter 20: Gene Therapy of X-Linked Severe Combined Immunodeficiency

Chapter 21: Gene Transfer for Generation of Tumor and Leukemia Vaccines

Chapter 22: Cytokine Gene Delivery into the Central Nervous System Using Intrathecally Injected Nonreplicative Viral Vectors

Chapter 23: Ex Vivo Cell Expansion

Chapter 24: In Vitro Regulation of Colony Stimulating Factor-Mediated Hematopoiesis in Healthy Individuals and Patients with Different Types of Myeloproliferative Disease

Chapter 25: In Vitro Generation of Dendritic Cells from Cord Blood CD34+ Hematopoietic Progenitor Cells

Chapter 26: Generation of Transgenic T Cells from Human CD34+ Cord Blood Cells

Chapter 27: Ex Vivo Expansion of Umbilical Cord Blood Cells on Feeder Layers

Chapter 28: Ex Vivo Production of Cord Blood CD34+ Derived Myeloid Precursors After Serum-Free Static Culture

Chapter 29: Ex Vivo Expansion of Hematopoietic Stem Cells

Chapter 30: Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use

Chapter 31: Cytokine-Mediated Expansion of Human NOD-SCID-Repopulating Cells

Chapter 32: Maturation and Differentiation of Dendritic Cells

Chapter 33: Differentiation of Human Antigen-Presenting Dendritic Cells from CD34+ Hematopoietic Stem Cells In Vitro

Chapter 34: Dendritic Cell Development from Mobilized Peripheral Blood CD34+ Cells

Chapter 35: In Vitro Maturation of Dendritic Cells from Blood Progenitors

Chapter 36: Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral Blood

Chapter 37: Preparation of Human Dendritic Cells for Tumor Vaccination

Chapter 38: Generation of Leukemic Dendritic Cells from Patients with Acute Myeloid Leukemia

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