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Cytoskeleton methods and protocols 4th Edition by Ray Gavin 9781071616604 1071616609

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Cytoskeleton methods and protocols Fourth Edition Ray H. Gavin (Editor) Digital Instant Download

Author(s): Ray H. Gavin (editor)
ISBN(s): 9781071616604, 1071616609
Edition: Fourth
File Details: PDF, 12.90 MB
Year: 2021
Language: english
SKU: EB-34830548 Category: Tag:

Cytoskeleton methods and protocols 4th Edition by Ray Gavin – Ebook PDF Instant Download/Delivery: 9781071616604, 1071616609

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Product details:

ISBN 10: 1071616609

ISBN 13: 9781071616604

Author: Ray H. Gavin

The fourth edition provides new and updated chapters that focus on experimental models, useful for investigating various aspects of cytoskeleton structure and function. Chapters detail protocols on animal models, plant models, protist models, fungal models, live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, genetics, and proteomics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Cytoskeleton: Methods and Protocols, Fourth Edition aims to provide an updated version to help researchers expand their understanding of cytoskeleton structure and function.

Table of contents:

  1. Imaging the Cytoskeleton

  2. Super-Resolution Imaging of the Actin Cytoskeleton in Living Cells Using TIRF-SIM

  3. Imaging Cytoskeleton Components by Electron Microscopy

  4. Imaging the Actin Cytoskeleton in Live Budding Yeast Cells

  5. Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells

  6. Imaging of Actin Cytoskeletal Integrity During Aging in C. elegans

  7. Imaging the Cytoskeleton in Living Plant Roots

  8. Imaging of Actin Cytoskeleton in the Nematode Caenorhabditis elegans

  9. Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells

  10. Influencing the Actin Dynamics in Plant Cells by Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins

  11. Analysis of Morphogenesis and Flagellar Assembly During Spermatogenesis in Planarian Flatworms

  12. An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells

  13. Purification of Cytoskeletal Proteins by Fast Protein Liquid Chromatography (FPLC) Using an ÄKTA Start System

  14. The Cilioprotist Cytoskeleton, a Model for Understanding How Cell Architecture and Pattern Are Specified

  15. 3D and 4D Tumorigenesis Model for the Quantitative Analysis of Cancer Cell Behavior and Screening for Anticancer Drugs

  16. Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells

  17. Chemotaxis: Under Agarose Assay

  18. Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila

  19. Using a Hand-Held Gene Gun for Genetic Transformation of Tetrahymena thermophila

  20. Proteomic Tools for the Analysis of Cytoskeleton Proteins

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Tags: Ray Gavin, Cytoskeleton, methods, protocols