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DNA and RNA Profiling in Human Blood Methods and Protocols 1st Edition by Peter Bugert 1934115932 9781934115930

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DNA and RNA Profiling in Human Blood Methods and Protocols 1st Edition Maryse St-Louis (Auth.) Digital Instant Download

Author(s): Maryse St-Louis (auth.), Peter Bugert (eds.)
ISBN(s): 9781934115930, 1934115932
Edition: 1
File Details: PDF, 8.87 MB
Year: 2009
Language: english
SKU: EB-1315580 Category: Tags: ,

DNA and RNA Profiling in Human Blood Methods and Protocols 1st Edition by Peter Bugert – Ebook PDF Instant Download/Delivery: 1934115932, 9781934115930

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Product details:

ISBN 10: 1934115932 

ISBN 13: 9781934115930

Author: Peter Bugert

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology(TM) series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.

Table of contents:

  1. PCR–ELISA for High-Throughput Blood Group Genotyping

  2. Single Base Extension in Multiplex Blood Group Genotyping

  3. Real-Time PCR Assays for High-Throughput Blood Group Genotyping

  4. Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing

  5. Multiplex ABO Genotyping by Minisequencing

  6. Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays

  7. Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance

  8. Multiplex HLA-Typing by Pyrosequencing

  9. High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach

  10. Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads

  11. The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping

  12. Nanoliter High-Throughput PCR for DNA and RNA Profiling

  13. RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis

  14. RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR

  15. cDNA Amplification by SMART-PCR and Suppression Subtractive Hybridization (SSH)-PCR

  16. Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE)

  17. Genome-Wide Platelet RNA Profiling in Clinical Samples

  18. PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples

  19. MicroRNA Profiling of Megakaryocytes

  20. Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes

  21. Real-Time PCR Analysis for Blood Cell Lineage Specific Markers

  22. Monitoring the Immune Response Using Real-Time PCR

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Tags: Peter Bugert, Profiling, DNA, Methods