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Embryonic Stem Cell Therapy for Osteo Degenerative Diseases Methods and Protocols 1st Edition by Nicole zur Nieden 1607619628 9781607619628

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Embryonic Stem Cell Therapy for Osteo Degenerative Diseases Methods and Protocols 1st Edition Nicole I. Zur Nieden (Auth.) Digital Instant Download

Author(s): Nicole I. zur Nieden (auth.), Nicole I. Nieden (eds.)
ISBN(s): 9781607619628, 1607619628
Edition: 1
File Details: PDF, 9.76 MB
Year: 2011
Language: english
SKU: EB-2448466 Category: Tags: ,

Embryonic Stem Cell Therapy for Osteo Degenerative Diseases Methods and Protocols 1st Edition by Nicole zur Nieden – Ebook PDF Instant Download/Delivery: 1607619628, 9781607619628

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Product details:

ISBN 10: 1607619628 

ISBN 13: 9781607619628

Author: Nicole I. zur Nieden

Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.

Table of contents:

  1. Embryonic Stem Cells for Osteo-Degenerative Diseases

  2. Methods for Culturing Mouse and Human Embryonic Stem Cells

  3. Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells

  4. Functional Assays for Human Embryonic Stem Cell Pluripotency

  5. Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells

  6. Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters

  7. Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives

  8. Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers

  9. Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology

  10. Methods for Embryoid Body Formation: The Microwell Approach

  11. Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview

  12. Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells

  13. Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the “Raclure” Method

  14. Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes

  15. Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds

  16. Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells

  17. Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield

  18. Identification of Osteoclasts in Culture

  19. Analysis of Glycosaminoglycans in Stem Cell Glycomics

  20. Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent

  21. Measurement and Illustration of Immune Interaction after Stem Cell Transplantation

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Tags: Nicole zur Nieden, Embryonic, Cell, Therapy