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Epidermal Cells Methods and Protocols 4th Edition by Kursad Turksen 1071602500 9781071602508

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Epidermal Cells 4th Edition Kursad Turksen Digital Instant Download

Author(s): Kursad Turksen
ISBN(s): 9781071602508, 1071602500
Edition: 4th
File Details: PDF, 9.24 MB
Year: 2020
Language: english
SKU: EB-47706514 Category: Tag:

Epidermal Cells Methods and Protocols 4th Edition by Kursad Turksen – Ebook PDF Instant Download/Delivery: 1071602500, 9781071602508

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Product details:

ISBN 10: 1071602500 

ISBN 13: 9781071602508

Author: Kursad Turksen

This fully updated edition brings together a new set of protocols to arm epidermal cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory mechanisms underlying epidermal cell development and function. The collection takes a detailed view at techniques currently being applied to this developing field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Epidermal Cells: Methods and Protocols, Fourth Edition serves as a valuable reference for experts as well as novices in the epidermal cell field.

Table of contents:

  1. The Simplest Protocol for Rapid and Long-Term Culture of Primary Epidermal Keratinocytes from Human and Mouse

  2. Dermal-Epidermal Separation by Heat

  3. Dermal-Epidermal Separation by Enzyme

  4. Dermal-Epidermal Separation by Chemical

  5. Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures

  6. Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential

  7. Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis

  8. Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte

  9. Digital Quantification of Epidermal Protein Expression in Paraffin-Embedded Tissue Using Immunohistochemistry

  10. Keratinocyte Differentiation by Flow Cytometry

  11. In Silico and In Vitro Considerations of Keratinocyte Nuclear Receptor Protein Structural Order for Improving Experimental Analysis

  12. Genetic Modification of Human Primary Keratinocytes by Lentiviral Vectors

  13. Generation of Knockout Human Primary Keratinocytes by CRISPR/Cas9

  14. In Vitro Wound Healing Assays to Investigate Epidermal Migration

  15. Iterative Three-Dimensional Epidermis Bioengineering and Xenografting to Assess Long-Term Regenerative Potential in Human Keratinocyte Precursor Cells

  16. Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse

  17. Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures

  18. Information and Statistical Analysis Pipeline for High-Throughput RNA Sequencing Data

  19. Cell-Extracellular Matrix Adhesion Assay

  20. Methods for Analysis of Keratinocyte Migration

  21. Scratch Wound Healing Assay

  22. Photoprotective Activity Assay Toward Ultraviolet B in Human Keratinocytes

  23. Photodynamic Therapy Assay

  24. A Method to Prepare Claudin-Modulating Recombinant Proteins

  25. Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application

  26. Ciliated Epithelial Cell Differentiation at Air-Liquid Interface Using Commercially Available Culture Media

  27. Correction to: Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures

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Tags: Kursad Turksen, Epidermal, Methods, Protocols