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Flow Cytometry First Principles 2nd Edition by Alice Longobardi Givan ISBN 0471382248 9780471382249

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Flow Cytometry First Principles 2nd Edition Alice Longobardi Givan Digital Instant Download

Author(s): Alice Longobardi Givan
ISBN(s): 0471382248
Edition: 2
File Details: PDF, 4.49 MB
Year: 2001
Language: english
SKU: EB-2167988 Category: Tag:

Flow Cytometry First Principles 2nd Edition by Alice Longobardi Givan – Ebook PDF Instant Download/Delivery: 0471382248, 9780471382249
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ISBN 10: 0471382248 
ISBN 13: 9780471382249
Author: Alice Longobardi Givan

Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis.

Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource:

  • Assumes no prior knowledge of flow cytometry
  • Progresses with an informal, engaging lecture style from simpleto more complex concepts
  • Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis
  • Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields

Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Flow Cytometry First Principles 2nd Table of contents:

Chapter 1: The Past as Prologue

Chapter 2: Setting the Scene

Chapter 3: Instrumentation: Into the Black Box

  • Illumination of the Stream

  • Centering Cells in the Illuminating Beam

  • Detection of Signals from Cells

  • Electronics

Chapter 4: Information: Harnessing the Data

  • Data Storage

  • Data Analysis

Chapter 5: Seeing the Light: Lasers, Fluorochromes, and Filters

  • General Theory

  • Lasers

  • Fluorochromes

  • Partitioning the Signal with Lenses, Filters, and Mirrors

  • Spectral Compensation

Chapter 6: Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating

  • Cells from Blood

  • Staining for Surface Markers

  • Controls

  • Quantitation

  • Sensitivity

  • The Strategy of Gating

  • Gating on Fluorescence

Chapter 7: Cells from Within: Intracellular Proteins

  • Methods for Permeabilizing Cells

  • Examples of Intracellular Staining

Chapter 8: Cells from Within: DNA in Life and Death

  • Fluorochromes for DNA Analysis

  • Ploidy

  • Cell Cycle Analysis

  • Two-Color Analysis for DNA and Another Parameter

  • Chromosomes

  • Apoptosis

  • Necrosis

Chapter 9: The Sorting of Cells

  • Sorting Theory

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Tags: Alice Longobardi Givan, Cytometry, Principles