Fluorescence Spectroscopy and Microscopy Methods and Protocols 1st Edition by Yves Engelborghs, Antonie Visser 9781627036498 1627036490

Fluorescence Spectroscopy and Microscopy Methods and Protocols 1st Edition by Yves Engelborghs, Antonie Visser – Ebook PDF Instant Download/Delivery: 9781627036498, 1627036490

Full download Fluorescence Spectroscopy and Microscopy Methods and Protocols 1st Edition after payment

 

Product details:

ISBN 10: 1627036490

ISBN 13: 9781627036498

Author: Yves Engelborghs, Antonie J.W.G. Visser

Reflecting the expanding field’s need for reliable protocols, __Fluorescence Spectroscopy and Microscopy: Methods and Protocols__ offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular __Methods in Molecular Biology__ series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
Comprehensive and practical, __Fluorescence Spectroscopy and Microscopy: Methods and Protocols__ aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Table of contents:

1. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra

2. Steady-State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions

3. Quantitative Fluorescence Spectral Analysis of Protein Denaturation

4. High-Pressure Fluorescence Applications

5. Frequency Domain Fluorometry: Theory and Application

6. Polar Plot Representation of Time-Resolved Fluorescence

7. Ensemble and Single-Molecule Detected Time-Resolved FRET Methods in Studies of Protein Conformations and Dynamics

8. MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes

9. Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins

10. Global Analysis of Time-Resolved Fluorescence Data

11. Nanometrology

12. Upconversion Spectrophotofluorometry

13. Subpicosecond Kerr-Gate Spectrofluorometry

14. Photoinduced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay

15. Biosynthetic Incorporation of Tryptophan Analogs in Proteins

16. Optimization of Fluorescent Proteins

17. Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family

18. Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope

19. Quantitative Protocol for Intensity-Based Live Cell FRET Imaging

20. Wide-Field Fluorescence Lifetime Imaging with Multi-Anode Detectors

21. Global Analysis of FRET-FLIM Data in Live Plant Cells

22. Time-Resolved Fluorescence Anisotropy Imaging

23. Multimodal Fluorescence Imaging Spectroscopy

24. Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells

25. Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells

26. Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS)

27. PET-FCS: Probing Rapid Structural Fluctuations of Proteins and Nucleic Acids by Single-Molecule Fluorescence Quenching

28. Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes

29. Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane

30. Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS

31. Quantitative Study of Protein-Protein Interactions in Live Cell by Dual-Color Fluorescence Correlation Spectroscopy

32. Brightness Experiments

33. Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy

34. Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy

35. Single-Molecule Fluorescence of Nucleic Acids

36. Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Circumventing the Diffraction Limit of Light

People also search:

methods of single molecule fluorescence spectroscopy and microscopy

what is fluorescence spectroscopy

what does fluorescence spectroscopy measure

fluorescence spectroscopy vs fluorescence microscopy

types of fluorescence spectroscopy

Tags: Yves Engelborghs, Antonie Visser, Fluorescence, Microscopy