Glycosyltransferases Methods and Protocols 1st Edition by Inka Brockhausen 162703465X 9781627034654

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ISBN 10: 162703465X 

ISBN 13: 9781627034654

Author: Inka Brockhausen

Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease.  Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize specific linkages between sugars or sugars and protein. This is in contrast to the non-specific or less specific chemical glycation reactions, transglycosylation and  reverse glycosylation reactions. Glycosyltransferases: Methods and Protocols contains a wide range of studies, methods and protocols which form a solid basis for investigations of the role and mechanisms, biology and pathology involving GTs. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Table of contents:

Glycan Microarray Screening Assay for Glycosyltransferase Specificities / W. Peng, C.M. Nycholat, and N. Razi
A Fluorescence-based Assay for Core 1 [beta]3Galactosyltransferase (T-synthase) Activity / T. Ju and R.D. Cummings
Structural and Biochemical Analysis of a Bacterial Glycosyltransferase / F. Zhu … [et al.]
Study of the Biological Functions of Mucin Type Core 3 O-glycans / S.H. Lee and M. Fukuda
Generation of Anti-sulfated Glycan Antibodies Using Sulfotransferase-deficient Mice / H. Kawashima
Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in Cog Complex Depleted Mammalian Cells / R.A. Willett, I.D. Pokrovskaya, and V.V. Lupashin
A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights Into the Sequence-function Relationships of Golgi-glycosyltransferases / D. Petit … [et al.]
Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans During Development / E. Tian, L. Zhang, and K.G. Ten Hagen
Photoaffinity Labeling of Protein O-mannosyltransferases of the Pmt1/Pmt2 Subfamily / M. Loibl and S. Strahl
Enzymatic Analysis of the Protein O-glycosyltransferase, Rumi, Acting Toward Epidermal Growth Factor-like (EGF) Repeats / H. Takeuchi and R.S. Haltiwanger
Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling / E.J. Kim and J.A. Hanover
Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase and Assay of Its Substrate, UDP-GlcNAc / T. Lefebvre … [et al.]
In Vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases / M.A. Musumeci, M.V. Ielmini, and M.F. Feldman
In Vitro UDP-sugar:Undecaprenyl-phosphate Sugar-1-phosphate Transferase Assay and Product Detection by Thin Layer Chromatography / K.B. Patel and M.A. Valvano
In Vitro O-antigen Ligase Assay / X. Ruan and M.A. Valvano
Functional Identification of Bacterial Glucosyltransferase Wbdn / Y. Gao, A. Vinnikova, and I. Brockhausen
Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains / P.L. DeAngelis
Methods for Measuring Class I Membrane-bound Hyaluronan Synthase Activity / P.H. Weigel, A.J. Padgett-McCue, and B.A. Baggenstoss
Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei / L. Izquierdo, M.L. Guther, and M.A. Ferguson
Mannose-6-phosphate: A Regulator of LLO Destruction / N. Gao and M.A. Lehrman
N-acetylglucosaminyltransferase (GnT) Assays Using Fluorescent Oligosaccharide Acceptor Substrates: GnT-III, IV, V, and IX (GnT-Vb) / S. Takamatsu … [et al.]
A Method for Determination of UDP-GlcNAc:GlcNAc1-6(GlcNAc[beta]1-2)Man[alpha]1-R [GlcNAc to Man] Beta1-4N-acetylglucosaminyltransferase VI Activity / T. Taguchi and N. Taniguchi
In Vitro Assays of Orphan Glycosyltransferases and Their Application to Identify Notch Xylosyltransferases / M.K. Sethi … [et al.]
In Vitro Folding of [beta]-1,4Galactosyltransferase and Polypeptide-[alpha]-N-acetylgalactosaminyltransferase from the Inclusion Bodies / B. Ramakrishnan and P.K. Qasba
An Assay for [alpha]1,6-Fucosyltransferase (Fut8) Activity Based on the HPLC Separation of a Reaction Product with Fluorescence Detection / H. Ihara … [et al.]
Immunodetection of Glycosyltransferases in Gastrointestinal Tissues / J. Gomes and C.A. Reis
Glycosyltransferases in Chemo-enzymatic Synthesis of Oligosaccharides / B. Tefsen and I. Van Die

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Tags: Inka Brockhausen, Glycosyltransferases, Methods, Protocols