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Guanylate Cyclase and Cyclic GMP Methods and Protocols 1st Edition by Michael Russwurm,Robert Lukowski ISBN 1627034595 9781627034593

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Guanylate Cyclase and Cyclic GMP Methods and Protocols 1st Edition Michael Russwurm Digital Instant Download

Author(s): Michael Russwurm, Corina Russwurm, Doris Koesling (auth.), Thomas Krieg, Robert Lukowski (eds.)
ISBN(s): 9781627034593, 1627034595
Edition: 1
File Details: PDF, 4.56 MB
Year: 2013
Language: english
SKU: EB-4290510 Category: Tags: , , , ,

Guanylate Cyclase and Cyclic GMP Methods and Protocols 1st Edition by Michael Russwurm, Robert Lukowski – Ebook PDF Instant Download/Delivery: 1627034595, 9781627034593

Full download Guanylate Cyclase and Cyclic GMP Methods and Protocols 1st Edition after payment

 

 

Product details:

ISBN 10: 1627034595 

ISBN 13: 9781627034593

Author: Michael Russwurm, Robert Lukowski

  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

Table of contents:

 

1. Historical and Future Perspectives on cGMP Signaling

1.1. The Past, the Present, and the Future

2. Enzymes and Assays in cGMP Signaling

2.1. cGMP-Dependent Protein Kinases (cGK)
2.2. Enzyme Assays for cGMP Hydrolyzing Phosphodiesterases
2.3. Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues

3. Imaging and Biosensors for cGMP Detection

3.1. Hyperspectral Imaging of FRET-Based cGMP Probes
3.2. Visualization of cGMP with cGi Biosensors
3.3. Advances and Techniques to Measure cGMP in Intact Cardiomyocytes
3.4. Real-Time Monitoring of the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells

4. Molecular and Cellular Approaches to cGMP Signaling

4.1. Methods for Identification of cGKI Substrates
4.2. Approaches for Monitoring PKG1α Oxidative Activation
4.3. Analysis of cGMP Signaling in Adipocytes

5. Genetic and Pharmacological Studies on cGMP Function

5.1. A Genetic Strategy for the Analysis of Individual Axon Morphologies in cGMP Signaling Mutant Mice
5.2. Receptor Binding Assay for NO-Independent Activators of Soluble Guanylate Cyclase
5.3. Direct Intrathecal Drug Delivery in Mice for Detecting In Vivo Effects of cGMP on Pain Processing

6. cGMP in the Nervous System and Disease Models

6.1. The Geisler Method: Tracing Activity-Dependent cGMP Plasticity Changes upon Double Detection of mRNA and Protein on Brain Slices
6.2. Detection of cGMP in the Degenerating Retina

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Michael Russwurm,Robert Lukowski,Guanylate Cyclase and,Cyclic GMP,Methods and Protocols