Handbook of assay development in drug discovery 1st Edition by Lisa Minor 1574444719 9781574444711

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Product details:

ISBN 10: 1574444719 

ISBN 13: 9781574444711

Author: Lisa K. Minor

The Handbook of Assay Development in Drug Discovery describes all the tools currently available for performing various assay techniques. Featuring troubleshooting advice for common problems from experienced assay developers, the vendor community, and scientists in the pharmaceutical industry, the book presents descriptions of methods, laboratory guidelines and protocols used to perform such methods, specific examples of each assay system, and troubleshooting tools. Designed as a guide to running an assay from start to finish, this is an ideal bench top companion for scientists involved in drug discovery screening, lead profiling, therapeutic target evaluation, and assay development and implementation.

Table of contents:

Chapter 1: Protein Kinases in Drug Discovery: Rationale, Success, and Challenge
Chapter 2: An Introduction to the Protein Tyrosine Phosphatase Gene Family and Screening Assay Development
Chapter 3: Time-Resolved Fluorescence Based Assays for Kinases
Chapter 4: Development of High-Throughput Screening Assays in Scintillating Microplates (FlashPlates) to Identify Inhibitors of Kinase Activity
Chapter 5: Development of High-Throughput Screening Assays for Kinase Drug Targets Using AlphaScreen Technology
Chapter 6: Homogeneous High-Throughput Screening (HTS) Assays for Serine/Threonine Kinases Using -Galactosidase Enzyme Fragment Complementation
Chapter 7: IMAP Assays for Protein Kinases
Chapter 8: A Homogeneous, Luminescent, High-Throughput, Versatile Assay for a Wide Range of Kinases
Chapter 9: Proteases as Drug Targets
Chapter 10: A Comparison of Homogeneous Bioluminescent and Fluorescent Methods for Protease Assays
Chapter 11: Scintillation Proximity Assay (SPA) Receptor Binding Assays
Chapter 12: Radioligand Binding Filtration Assay: Full Automation
Chapter 13: Nuclear Receptors as Drug Targets
Chapter 14: Nuclear Receptor Scintillation Proximity Assays
Chapter 15: Homogeneous Assay Development for Nuclear Receptor Using AlphaScreen™ Technology
Chapter 16: Development of Nuclear Receptor Homogeneous Assay Using the Lance™ Technology
Chapter 17: The Emerging Role of Cell-Based Assays in Drug Discovery
Chapter 18: The Preparation of Cells for High-Content Screening
Chapter 19: The Evolution of cAMP Assays
Chapter 20: A Homogeneous, Fluorescent Polarization Assay for Inositol 1,4,5-Trisphosphate (Ins P3)
Chapter 21: Scintillation Proximity Assay of Inositol Phosphates
Chapter 22: Measuring Calcium Mobilization with G -Coupled GPCRs Using the Fluormetric Imaging Plate Reader (FLIPR)
Chapter 23: Development of FLIPR-Based HTS Assay for G;-Coupled GPCRs
Chapter 24: Aurora Assays
Chapter 25: Membrane Potential Based Assays for Ion Channels and Electrogenic Transporters
Chapter 26: Reporter Gene Assays for Drug Discovery
Chapter 27: mRNA Detection from Cells Using Quantigene® Branched DNA Technology
Chapter 28: Homogeneous Multiwell Assays for Measuring Cell Viability, Cytotoxicity, and Apoptosis
Chapter 29: Development of Image-Based Assays for Drug Discovery
Chapter 30: GPCR Internalization Measured by Image Analysis
Chapter 31: TRANSFLUOR Provides a Universal Cell-Based Assay for Screening G-Protein-Coupled Receptors

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Tags: Lisa Minor, assay, development, discovery