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HDAC HAT Function Assessment and Inhibitor Development Methods and Protocols 1st Edition by Oliver Krämer 9781493965250 1493965255

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HDAC HAT Function Assessment and Inhibitor Development 1st Edition Oliver H. Krämer Digital Instant Download

Author(s): Oliver H. Krämer
ISBN(s): 9781493965250, 1493965255
Edition: 1st
File Details: PDF, 13.31 MB
Year: 2016
Language: english
SKU: EB-47740620 Category: Tag:

HDAC HAT Function Assessment and Inhibitor Development Methods and Protocols 1st Edition by Oliver Krämer – Ebook PDF Instant Download/Delivery: 9781493965250, 1493965255

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Product details:

ISBN 10: 1493965255

ISBN 13: 9781493965250

Author: Oliver H. Krämer

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study.

Table of contents:

  1. Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells

  2. Assessment of HDACi-Induced Protein Cleavage by Caspases

  3. Assessment of HDACi-Induced Cytotoxicity

  4. Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization

  5. Analysis of Histone Deacetylase-Dependent Effects on Cell Migration Using the Stripe Assay

  6. Using Histone Deacetylase Inhibitors to Analyze the Relevance of HDACs for Translation

  7. Effects of HDACi on Immunological Functions

  8. DNA Fiber Spreading Assay to Test HDACi Effects on DNA and Its Replication

  9. Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination

  10. Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemia Cells for HDAC Inhibitor Research

  11. Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes via Proximity Ligation Assay

  12. Purification and Analysis of Male Germ Cells from Adult Mouse Testis

  13. Generation of Tissue-Specific Mouse Models to Analyze HDAC Functions

  14. Engineering of Conditional Class I Hdac Knockout Mice and Generation of a Time-Spatial Knockout by a Dual Recombination System

  15. Xenografting of Cancer Cell Lines for In Vivo Screening of the Therapeutic Potential of HDAC Inhibitors

  16. Generation of Xenotransplants from Human Cancer Biopsies to Assess Anti-Cancer Activities of HDACi

  17. Phospho-Flow Analysis of Primary Mouse Cells after HDAC Inhibitor Treatment

  18. Evaluation of Innate Immune Gene Expression Following HDAC Inhibitor Treatment by High-Throughput qPCR and PhosFlow Cytometry

  19. Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP)

  20. Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases

  21. Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells

  22. Testing the Effects of SIAH Ubiquitin E3 Ligases on Lysine Acetyl Transferases

  23. Assessment of HDACi-Induced Acetylation of Non-Histone Proteins by Mass Spectrometry

  24. Assessing the Role of Paralog-Specific Sumoylation of HDAC1

  25. Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques

  26. How to Distinguish Between the Activity of HDAC1–3 and HDAC6 with Western Blot

  27. Evaluating the Effect of HDAC8 Inhibition in Malignant Peripheral Nerve Sheath Tumors

  28. Analyzing the Impact of Pan- and Class-Specific HDACi on Differentiation-Associated Factors

  29. Encapsulation of the HDACi Ex527 into Liposomes and Polymer-Based Particles

  30. Preparation of Histone Deacetylase Inhibitor Vorinostat Loaded Poly(D,L-lactide-co-glycolide) Polymeric Nanoparticles by Nanoprecipitation Method

  31. Generation and Assessment of Fusions Between HDACi and TKi

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Tags: Oliver Krämer, Function, Assessment, Inhibitor