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Lectins Methods and Protocols 1st Edition by Jun Hirabayashi 9781493912926 1493912925

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Lectins Methods and Protocols 1st Edition Jun Hirabayashi (Eds.) Digital Instant Download

Author(s): Jun Hirabayashi (eds.)
ISBN(s): 9781493912926, 1493912925
Edition: 1
File Details: PDF, 18.79 MB
Year: 2014
Language: english
SKU: EB-4930058 Category: Tag:

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Product details:

ISBN 10: 1493912925

ISBN 13: 9781493912926

Author: Jun Hirabayashi

Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but alsonon-experts including both young scientists.

Table of contents:

PART I. STANDARD TECHNIQUES
Chapter 1: History of Plant Lectin Research
Chapter 2: Fungal Lectins: A Growing Family
Chapter 3: The “White Kidney Bean Incident” in Japan
Chapter 4: Hemagglutination (Inhibition) Assay
Chapter 5: Preparation of Affinity Adsorbents and Purification of Lectins from Natural Sources
Chapter 6: High-Performance Lectin Affinity Chromatography
Chapter 7: Determination of Glycan Motifs Using Serial Lectin Affinity Chromatography
Chapter 8: Lectin-Probed Western Blot Analysis
Chapter 9: Solid-Phase Assay of Lectin Activity Using HRP-Conjugated Glycoproteins
Chapter 10: A Simple Viral Neuraminidase-Based Detection for High-Throughput Screening of Viral Hemagglutinin-Host Receptor Specificity
Chapter 11: Lectin Affinity Electrophoresis
Chapter 12: Capillary-Based Lectin Affinity Electrophoresis for Interaction Analysis Between Lectins and Glycans
Chapter 13: Basic Procedures for Lectin Flow Cytometry
Chapter 14: Histochemical Staining Using Lectin Probes
Chapter 15: Equilibrium Dialysis Using Chromophoric Sugar Derivatives
Chapter 16: Centrifugal Ultrafiltration-HPLC Method for Interaction Analysis Between Lectins and Sugars
Chapter 17: Surface Plasmon Resonance as a Tool to Characterize Lectin-Carbohydrate Interactions
Chapter 18: Isothermal Calorimetric Analysis of Lectin-Sugar Interaction
Chapter 19: Carbohydrate-Lectin Interaction Assay by Fluorescence Correlation Spectroscopy Using Fluorescence-Labeled Glycosylasparagines

PART II. EMERGING TECHNIQUES FOR LECTIN-BASED GLYCOMICS
Chapter 20: Lectin-Based Glycomics: How and When Was the Technology Born?
Chapter 21: Frontal Affinity Chromatography (FAC): Theory and Basic Aspects
Chapter 22: Frontal Affinity Chromatography: Practice of Weak Interaction Analysis Between Lectins and Fluorescently Labeled Oligosaccharides
Chapter 23: Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation
Chapter 24: Lectin-Microarray Technique for Glycomic Profiling of Fungal Cell Surfaces
Chapter 25: Application of Lectin Microarray to Bacteria Including Lactobacillus casei/paracasei Strains
Chapter 26: Live-Cell Imaging of Human Pluripotent Stem Cells by a Novel Lectin Probe rBC2LCN
Chapter 27: Carbohydrate-Binding Specificity of Lectins Using Multiplexed Glyco-Bead Array
Chapter 28: Supported Molecular Matrix Electrophoresis: A New Membrane Electrophoresis for Characterizing Glycoproteins

PART III. TECHNIQUES FOR ELUCIDATING FUNCTIONS OF ENDOGENOUS ANIMAL LECTINS
Chapter 29: Overall Strategy for Functional Analysis of Animal Lectins
Chapter 30: Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System
Chapter 31: Potential Usage for In Vivo Lectin Screening in Live Animals Utilizing Cell Surface Mimetic Glyco-nanoparticles, Phosphorylcholine-Coated Quantum Dots (PC-QDs)
Chapter 32: Remodeling Cell Surface Glycans Using Glycosylation Inhibitors
Chapter 33: Remodeling of Glycans Using Glycosyltransferase Genes
Chapter 34: Functional Assay Using Lectin Gene Targeting Technologies (Over-Expression)
Chapter 35: Analysis of L-Selectin-Mediated Cellular Interactions Under Flow Conditions
Chapter 36: Assessment of Weak Sugar-Binding Ability Using Lectin Tetramer and Membrane-Based Glycans

PART IV. STRUCTURAL BIOLOGY AND ENGINEERING OF LECTINS
Chapter 37: Perspectives in Glycomics and Lectin Engineering
Chapter 38: Molecular Basis of a Pandemic of Avian-Type Influenza Virus
Chapter 39: Basic Procedure of X-Ray Crystallography for Analysis of Lectin-Sugar Interactions
Chapter 40: A New Structure Determination Method of Lectins Using a Selenium-Containing Sugar Ligand
Chapter 41: NMR Analysis of Carbohydrate-Binding Interactions in Solution: An Approach Using Analysis of Saturation Transfer Difference NMR Spectroscopy
Chapter 42: Small-Angle X-Ray Scattering to Obtain Models of Multivalent Lectin-Glycan Complexes
Chapter 43: Directed Evolution of Lectins by an Improved Error-Prone PCR and Ribosome Display Method
Chapter 44: Tracing Ancestral Specificity of Lectins: Ancestral Sequence Reconstruction Method as a New Approach in Protein Engineering

PART V. COMPREHENSIVE LISTS
Chapter 45: Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities
Chapter 46: Lectin Structures: Classification Based on the 3-D Structures

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Tags: Jun Hirabayashi, Lectins, Methods, Protocols