Manual of Diagnostic Cytology of the Dog and Cat 1st Edition by John Dunn 0470658703 9780470658703

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ISBN 10: 0470658703

ISBN 13: 9780470658703

Author: John Dunn

Wish you could interpret cytological specimens in practice rather than paying a lab to do it for you?  Want to provide your clients with a faster service? Manual of Diagnostic Cytology of the Dog and Cat is the ideal quick reference for the busy veterinarian in first opinion practice. It describes techniques for obtaining good quality cytological diagnostic specimens, and guides you through the interpretation of cytological findings. Created to be used alongside the microscope, hundreds of high quality colour photos will help you to identify normal cell types and abnormal cytology, including both non-neoplastic and neoplastic lesions. It describes in a clear and concise manner the most common lesions and related disorders encountered in a practice setting. The concise format means that you can quickly find exactly what you’re looking for. Covering indications for cytological investigation, collection techniques and the evaluation and interpretation of findings, this concise manual will be your go-to resource.

Manual of Diagnostic Cytology of the Dog and Cat 1st Table of contents:

1 Cytological Collection Techniques and Sample Preparation

Sampling techniques

Fine-needle aspiration

Impression smears/imprints

Scrapings

Swab smears

Brushings

Collection and handling of fluid samples for cytological examination

Staining techniques

Microscopic examination of cytological specimens

Recognition of artefacts

2 General Principles of Cytological Interpretation

Initial assessment of the sample: Is the preparation of diagnostic quality?

Inflammation

Neutrophilic inflammation

Macrophage-rich or granulomatous inflammation

Eosinophilic inflammation

Lymphocytic/plasmacytic inflammation

Mixed inflammation

Aspiration of normal or hyperplastic tissue

Neoplasia

Criteria for malignancy

Dysplasia

Characteristics of cell populations: Epithelial, mesenchymal or discrete round cell?

Epithelial

Mesenchymal

Discrete round cells

Cystic structures

3 Cytology of the Lymphoid Tissues

The lymph node

Normal cytological appearance and benign changes

Non-lymphoid tissue

Reactive hyperplasia

Lymphadenitis

N eoplasia

Metastatic malignancies

Lymphoid malignancies

Morphology of canine lymphomas classified by the Kiel classification

Histiocytic sarcoma

The spleen

Benign changes

Neoplasia

Thymus

4 Cytology of Cutaneous and Subcutaneous Lesions

Introduction

Normal cell types

Non-neoplastic lesions

Neoplastic lesions

Epithelial tumours

Mesenchymal tumours

Round cell tumours

5 Cytology of the Respiratory Tract

Introduction

Collection techniques

Nasal flush

Transtracheal wash and bronchoalveolar lavage

Transtracheal wash

Bronchoalveolar lavage

Transthoracic fine-needle aspiration

Cytological evaluation

Nasal cytology

Normal cell types

Inflammation/infection

Neoplasia

Cytology of the trachea and bronchial tree

Normal cell types

Mucus

Oropharyngeal contamination

Inflammation

Eosinophilic inflammation

Dysplastic epithelial cells

Squamous metaplasia

Intrapulmonary haemorrhage

Neoplasia (primary or metastatic)

6 Biochemical and Cytological Evaluation of Body Cavity Effusions

Introduction

Sample collection and processing

Classification of effusions

Traditional system

Aetiological system

Artefacts

Conclusion

Acknowledgment

References

7 Cytology of Synovial Fluid

Introduction

Collection

Restraint

Asepsis

Equipment

Approach

Sample handling

Synovial fluid properties

Volume

Colour and turbidity

Viscosity

Mucin quality

Total nucleated cell count

Total protein

Cytology

Culture

Ancillary diagnostic tests

Antinuclear antibodies (ANA)

Rheumatoid factor

Classification of joint disease

Inflammatory arthropathies

Infectious arthritis

Non-infectious arthritis

Degenerative joint disease

Haemarthrosis

Neoplasia

8 Biochemical and Cytological Examination of Cerebrospinal Fluid

Contraindications

CSF collection sites and technique

Processing of CSF

Gross appearance

CSF protein concentration

Cell counts

CSF cytology

Haemorrhage

Non-inflammatory cells

Neutrophilic pleocytosis

Mononuclear pleocytosis

Eosinophilic pleocytosis

Myelin

Additional testing

9 Cytology of the Eye and Adnexal Structures

Eyelids

Indications for cytological investigation

Collection techniques

Normal cell types

Abnormal cytology

Other infectious agents

Neoplasia

Conjunctiva and cornea

Indications for cytological investigation

Collection techniques

Normal cell types

Abnormal cytology

Other infectious agents

Neoplasia

Anterior chamber

Collection technique

Indications for cytological investigation

Normal cell types

Abnormal cytology

Vitreous

Normal cell types

Orbit

Collection techniques

Normal cell types

Abnormal cytology

10 Cytology of the Urinary Tract

Collection techniques

Urine

Bladder and urethra

Kidneys

Sample preparation

Cytological evaluation

Urine sediment cytology

Cytology of bladder washes, fine-needle aspirates and impression smears

Renal cytology

Abnormal cytology

Inflammation

Neoplasia

11 Cytology of the Liver, Exocrine Pancreas and Gastrointestinal Tract

Liver

Normal cytological findings

Non-neoplastic disorders

Neoplasia

The exocrine pancreas

Normal cytological findings

Non-neoplastic disorders

Neoplasia

Gastrointestinal tract

Stomach

Intestines

References

12 Cytological Examination of the Endocrine Glands

Introduction

Cytology of the thyroid gland

Cytology of the adrenal glands

Cytology of the endocrine pancreas

References

13 Cytology of the Male and Female Genital Tracts

Introduction

Cytology of the male genital tract

Disorders of the testicle and scrotum

Infectious orchitis and epididymitis

Testicular tumours

Disorders of the prostate

Normal cell types

Benign prostatic hyperplasia

Prostatic and paraprostatic cysts

Prostatitis and prostatic abscess

Prostatic neoplasms

Squamous metaplasia

Disorders of the penis and prepuce

Balanitis and posthitis

Penile and preputial neoplasms

Semen collection and evaluation

Common conditions affecting semen quality

Cytology of the female genital tract

Infectious and inflammatory disorders

Neoplastic disorders

Vaginal cytology for breeding management

Ovarian remnant syndrome

References

14 Cytology of Mammary Gland Lesions

Indications for cytological investigation

Collection techniques

Samples from mammary lesions

Mammary secretion

Normal cell types and cytological appearance of normal lactating mammary gland

Mastitis

Feline mammary fibroadenomatous hyperplasia

Solid benign lesions (adenoma, canine lobular hyperplasia)

Benign mixed and complex mammary tumours

Mammary cysts

Malignant epithelial neoplasms

Mammary sarcomas

Malignant mixed mammary tumours

15 Cytology of Selected Infectious Organisms

Introduction

Bacterial infections

Fungal infections

Protozoal infections

Parasitic infections

Viral infections

Further Reading

General

Collection Techniques and Principles of Interpretation

Respiratory Tract Cytology

Gastrointestinal Tract Cytology

Endocrine Cytology

Cytology of the Lymphoid System

Cerebrospinal Fluid

Genital Tract Cytology

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