Mass Spectrometry of Proteins and Peptides Methods and Protocols 2nd Edition by Mary Lipton, Ljiljana Paša Tolic 9781934115480

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ISBN 13: 9781934115480

Author: Mary S. Lipton, Ljiljana Paša-Tolic

When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.

Mass Spectrometry of Proteins and Peptides Methods and Protocols 2nd Table of contents:

Generation and Analysis of Multidimensional Protein Identification Technology Datasets

Quantitative Peptide and Protein Profiling by Mass Spectrometry

Label-Free Relative Quantitation of Prokaryotic Proteomes Using the Accurate Mass and Time Tag Approach

Classical Proteomics: Two-Dimensional Electrophoresis/MALDI Mass Spectrometry

The Use of Difference In-Gel Electrophoresis for Quantitation of Protein Expression

Liquid-Chromatography-Mass Spectrometry of Thylakoid Membrane Proteins

High Accuracy Mass Spectrometry in Large-Scale Analysis of Protein Phosphorylation

Manual Validation of Peptide Sequence and Sites of Tyrosine Phosphorylation from MS/MS Spectra

Assigning Glycosylation Sites and Microheterogeneities in Glycoproteins by Liquid Chromatography/Tandem Mass Spectrometry

Structure Analysis of N-Glycoproteins

Capillary Zone Electrophoresis-Mass Spectrometry for the Characterization of Isoforms of Intact Glycoproteins

Top-Down Proteomics on a High-field Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Capillary Isoelectric Focusing/Reversed Phase Liquid Chromatography/Mass Spectrometry

Integrating Accelerated Tryptic Digestion into Proteomics Workflows

Hydrogen/Deuterium Exchange Mass Spectrometry

Mass Spectrometry Detection and Characterization of Noncovalent Protein Complexes

Chemical Cross-Linking for Protein–Protein Interaction Studies

Tissue Analysis with High-Resolution Imaging Mass Spectrometry

Proteomic Global Profiling for Cancer Biomarker Discovery

Analysis of Protein Glycosylation and Phosphorylation Using Liquid Phase Separation, Protein Microarray Technology, and Mass Spectrometry

Transthyretin Mass Determination for Detection of Transthyretin Familial Amyloid

Characterization of Microorganisms by MALDI Mass Spectrometry

Mass Spectrometric Characterization of Neuropeptides

Peptide and Protein Ion/Ion Reactions in Electrodynamic Ion Traps: Tools and Methods

Electron Capture Dissociation LC/MS/MS for Bottom-Up Proteomics

Two-Dimensional Ion Mobility Analyses of Proteins and Peptides

Proteomics for Validation of Automated Gene Model Predictions

Support Vector Machines for Improved Peptide Identification from Tandem Mass Spectrometry Database Search

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Mary Lipton,Ljiljana Paša Tolic,Spectrometry,Proteins