Methods in Methane Metabolism Part A Methanogenesis 1st Edition by Amy Rosenzweig, Stephen Ragsdale 9780123851123 0123851122
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Product details:
ISBN 10: 0123851122
ISBN 13: 9780123851123
Author: Amy C. Rosenzweig, Stephen W. Ragsdale
Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.
Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field
Table of contents:
Chapter 1: Techniques for Cultivating Methanogens
Chapter 2: Genetic Methods for Methanosarcina Species
Chapter 3: Genetic Systems for Hydrogenotrophic Methanogens
Chapter 4: Molecular Tools for Investigating ANME Community Structure and Function
Chapter 5: Studying Gene Regulation in Methanogenic Archaea
Chapter 6: Growth of Methanogens Under Defined Hydrogen Conditions
Chapter 7: Preparation of [Fe]-Hydrogenase from Methanogenic Archaea
Chapter 8: Assay of Methylotrophic Methyltransferases from Methanogenic Archaea
Chapter 9: Methyl-Coenzyme M Reductase from Methanothermobacter Marburgensis
Chapter 10: Methods for Analysis of Acetyl-CoA Synthase: Applications to Bacterial and Archaeal Syst
Chapter 11: Acetate Kinase and Phosphotransacetylase
Chapter 12: Sodium Ion Translocation and ATP Synthesis in Methanogens
Chapter 13: Proton Translocation in Methanogens
Chapter 14: Measuring Isotope Fractionation by Autotrophic Microorganisms and Enzymes
Chapter 15: 2-Oxoacid Metabolism in Methanogenic CoM and CoB Biosynthesis
Chapter 16: Biomethanation and Its Potential
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Tags: Amy Rosenzweig, Stephen Ragsdale, Methods, Methanogenesis