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Metastasis Research Protocols 2nd Edition by Miriam Dwek, Udo Schumacher, Susan Brooks 1461482437 9781461482437

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Metastasis Research Protocols 2nd ed. 2014 Edition Edition Miriam Dwek Digital Instant Download

Author(s): Miriam Dwek, Udo Schumacher, Susan A. Brooks
ISBN(s): 9781461482437, 1461482437
Edition: 2nd ed. 2014 Edition
File Details: PDF, 4.42 MB
Year: 2013
Language: english
SKU: EB-47757328 Category: Tags: , ,

Metastasis Research Protocols 2nd Edition by Miriam Dwek, Udo Schumacher, Susan Brooks – Ebook PDF Instant Download/Delivery: 1461482437, 9781461482437

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ISBN 10: 1461482437

ISBN 13: 9781461482437

Author: Miriam Dwek, Udo Schumacher, Susan A. Brooks

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases.  The second edition of Metastasis Research Protocols, brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of  the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

Metastasis Research Protocols 2nd Table of contents:

In Vitro Invasion Assay Using Matrigel™: A Reconstituted Basement Membrane Preparation

Single Cell and Spheroid Collagen Type I Invasion Assay

Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells

Small-Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin Under Physiological Flow Conditions

Adhesion of Tumor Cells to Matrices and Endothelium

Cell Aggregation Assays

Chick Heart Invasion Assay

Computer Simulation of the Metastatic Progression

Theoretical Considerations in Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice

Syngeneic Murine Metastasis Models: B16 Melanoma

Imageable Clinically Relevant Mouse Models of Metastasis

Imaging Metastatic Cell Trafficking at the Cellular Level In Vivo with Fluorescent Proteins

Ultrasound Techniques for the Detection of Tumors and Metastases in Small Animals

The PFP/RAG2 Double-Knockout Mouse in Metastasis Research: Small-Cell Lung Cancer and Prostate Cancer

Ultrasound-Guided Intracardial Injection and In Vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Hematogenous Metastases

Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer

Spontaneous and Experimental Metastasis Models: Nude Mice

Identifying the Origin and Phenotype of Cells in Tumor Xenografts

Erratum

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Tags: Miriam Dwek, Udo Schumacher, Susan Brooks, Metastasis