Sale!

Mitochondria Methods in Cell Biology Volume 65 1st Edition by Liza Pon, Eric Schon 9780125612852 0125612850

Original price was: $50.00.Current price is: $35.00.

Mitochondria 1st Edition Liza A. Pon Digital Instant Download

Author(s): Liza A. Pon, Eric A. Schon (Eds.)
ISBN(s): 9780125612852, 0125612850
Edition: 1st
File Details: PDF, 12.57 MB
Year: 2001
Language: english
SKU: EB-1946038 Category: Tags: ,

Mitochondria Methods in Cell Biology Volume 65 1st Edition by Liza Pon, Eric Schon – Ebook PDF Instant Download/Delivery: 9780125612852, 0125612850

Full download Mitochondria Methods in Cell Biology Volume 65 1st Edition after payment

 

Product details:

ISBN 10: 0125612850

ISBN 13: 9780125612852

Author: Liza A. Pon, Eric A. Schon

This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs.

The book is full of step-by-step “how to” methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies.

  • Isolation of mitochondria from commonly used cells and tissues
  • Assays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeleton
  • Biochemical and optical methods for studying protein-protein interactions in mitochondria
  • Approaches to studying mitochondrial replication, transcription, and translation
  • Transmitochondrial technologies
  • Methods in microassay data analysis

Table of contents:

  1. Isolation and Subfractionation of Mitochondria from Animal Cells and Tissue Culture Lines

  2. Isolation and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae

  3. Isolation and Subfractionation of Mitochondria from Plants

  4. Assessing Functional Integrity of Mitochondria in Vitro and in Vivo

  5. Assaying Mitochondrial Respiratory Complex Activity in Mitochondria Isolated from Human Cells and Tissues

  6. In Vivo Measurements of Respiration Control by Cytochrome c Oxidase and in Situ Analysis of Oxidative Phosphorylation

  7. Assay of Mitochondrial ATP Synthesis in Animal Cells

  8. Measurement of Membrane Permeability and Permeability Transition of Mitochondria

  9. Assaying Actin-Binding Activity of Mitochondria in Yeast

  10. Analysis and Prediction of Mitochondrial Targeting Peptides

  11. Assaying Protein Import into Mitochondria

  12. Analysis of Protein–Protein Interactions in Mitochondria by Coimmunoprecipitation and Chemical Cross-Linking

  13. Blue-Native Gels to Isolate Protein Complexes from Mitochondria

  14. Application of Electron Tomography to Mitochondrial Research

  15. Epitope Tagging and Visualization of Nuclear-Encoded Mitochondrial Proteins in Yeast

  16. Targeting of Green Fluorescent Protein to Mitochondria

  17. Assessment of Mitochondrial Membrane Potential in Situ Using Single Potentiometric Dyes and Novel Fluorescence Resonance Energy

  18. Optical Imaging Techniques (Histochemical, Immunohistochemical, and in Situ Hybridization Staining Methods) to Visualize Mitochondria

  19. Visualization of Mitochondrial Movement in Yeast

  20. Targeting of Reporter Molecules to Mitochondria to Measure Calcium, ATP, and pH

  21. Genetic Transformation of Saccharomyces cerevisiae Mitochondria

  22. Transmitochondrial Technology in Animal Cells

  23. Diagnostic Assays for Defects in Mitochondrial DNA Replication and Transcription in Yeast and Human Cells

  24. Analysis of Mitochondrial Translation Products in Vivo and in Organello in Yeast

  25. Numerical Methods for Handling Uncertainty in Microarray Data: An Example Analyzing Perturbed Mitochondrial Function in Yeast

People also search:

mitochondria cells function

mitochondria cell meaning

biology mitochondrion

biology mitochondria definition

biology mitochondria

Tags: Liza Pon, Eric Schon, Mitochondria, Biology