Molecular Biomethods Handbook 2nd Edition by John Walker, Ralph Rapley 9781603273756 1603273751

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Product details:

ISBN 10: 1603273751

ISBN 13: 9781603273756

Author: John M. Walker, Ralph Rapley

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR

Table of contents:

1. Nucleic acid methods: The manipulation of nucleic acids: basic tools and techniques

2. Restriction enzymes: tools in clinical research

3. Principles and medical applications of the polymerase chain reaction

4. Probe design, production, and applications

5. Southern blotting as a diagnostic method

6. Capillary electrophoresis of DNA: biomedical applications

7. Denaturing high-performance liquid chromatography (DHPLC) for nucleic acid analysis

8. Denaturing gradient gel electrophoresis (DGGE)

9. Single strand conformation polymorphism (SSCP) analysis

10. Randomly amplified polymorphic DNA (RAPD): a useful tool for genomic characterization of different organisms

11. Quantification of mRNA using real time RT-PCR: the SYBR solution

12. Quantitative analysis of DNA sequences by PCR and solid-phase minisequencing

13. Multiplex amplifiable probe hybridization (MAPH)

14. Gene expression profiling

15. Comparative genomic hybridization in clinical and medical research

16. Subtractive hybridization

17. Fluorescence in situ hybridization

18. Quantitative trait locus mapping to identify genes for complex traits in mice

19. cDNA microarrays

20. Mapping techniques

21. Single nucleotide polymorphisms

22. Gene/protein sequence analysis: a compilation of bioinformatic tools

Protein and cell methods:
23. Protein electrophoresis
24. Protein blotting
25. Capillary electrophoresis of proteins
26. Autoradiograph and fluorography
27. Mass spectrometry of proteins and peptides
28. Post-translational modifications of proteins
29. Protein microarray technology
30. Protein-protein interactions
31. Glycoprotein analysis
32. Solid-phase peptide synthesis
33. Monoclonal antibodies
34. Antibody phage display
35. Protein engineering
36. Directed protein evolution
37. Enzyme linked immunosorbent assay (ELISA)
38. Epitope mapping: identification of antibody-binding sites on protein antigens
39. Quantum dots
40. Ion-exchange chromatography
41. Size-exclusion chromatography
42. Hydrophobic interaction chromatography
43. Affinity chromatography
44. High performance liquid chromatography (HPLC) of peptides and proteins
45. Amino acid analysis
46. Surface plasmon resonance
47. Macromolecular crystallography
48. Microchip devices for bioanalysis
49. Mammalian cell culture
50. Plant tissue culture
51. Stem cells and regenerative medicine
52. Cryopreservation: conservation of bioresources at ultra low temperatures
53. Magnetic resonance imaging
54. Electron microscopy
55. Confocal microscopy
56. Laser microdissection
57. Biomedical uses of flow cytometry
58. Immunomicroscopy
59. In situ hybridization
60. High throughput screening

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Tags: John Walker, Ralph Rapley, Molecular, Biomethods