Neisseria meningitidis Advanced Methods and Protocols 1st Edition by Myron Christodoulides 9781617793462 1617793469
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ISBN 10: 1617793469
ISBN 13: 9781617793462
Author: Myron Christodoulides
Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors. Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Neisseria meningitidis: Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.
Table of contents:
Chapter 1: Neisseria meningitidis: Biology, Microbiology, and Epidemiology
Chapter 2: Classification and Pathogenesis of Meningococcal Infections
Chapter 3: Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology
Chapter 4: Generating Knock-Out and Complementation Strains of Neisseria meningitidis
Chapter 5: Identification and Functional Characterization of sRNAs in Neisseria meningitidis
Chapter 6: Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins
Chapter 7: Genome-Scale Metabolic Models: Reconstruction and Analysis
Chapter 8: TMT Labelling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome
Chapter 9: Meningococcal Ligands and Molecular Targets of the Host
Chapter 10: In Vivo Imaging of Meningococcal Disease Dynamics
Chapter 11: Methods for Studying Neisseria meningitidis Biofilms
Chapter 12: A Laminar-Flow Chamber Assay for Measuring Bacterial Adhesion Under Shear Stress
Chapter 13: Techniques to Measure Pilus Retraction Forces
Chapter 14: Human Dendritic Cell Culture and Bacterial Infection
Chapter 15: Hydrogen–Deuterium Exchange Coupled to Mass Spectrometry to Investigate Ligand–Receptor Interactions
Chapter 16: Visualising PAMP–PRR Interactions Using Nanoscale Imaging
Chapter 17: Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells
Chapter 18: The Use of the Pan-Neisseria Microarray and Experimental Design for Transcriptomics Studies of Neisseria
Chapter 19: Analysis of Parameters Associated with Prevention of Cellular Apoptosis by Pathogenic Neisseriae and Purified Porins
Chapter 20: Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach
Chapter 21: Antigen Identification Starting from the Genome: A “Reverse Vaccinology” Approach Applied to MenB
Chapter 22: A DNA Vaccine Strategy for Effective Antibody Induction to Pathogen-Derived Antigens
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Tags: Myron Christodoulides, Neisseria, meningitidis, Advanced