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NF kappa B Methods and Protocols 1st Edition by Michael May 9781493924219 1493924214

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NF kappa B Methods and Protocols 1st Edition Michael J. May (Eds.) Digital Instant Download

Author(s): Michael J. May (eds.)
ISBN(s): 9781493924219, 1493924214
Edition: 1
File Details: PDF, 19.90 MB
Year: 2015
Language: english
SKU: EB-4985950 Category: Tag:

NF kappa B Methods and Protocols 1st Edition by Michael May – Ebook PDF Instant Download/Delivery: 9781493924219, 1493924214

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Product details:

ISBN 10: 1493924214

ISBN 13: 9781493924219

Author: Michael J. May

Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, NF-Kappa B: Methods and Protocols provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease.

Table of contents:

  1. Electrophoretic Mobility Shift Assay Analysis of NF-κB DNA Binding

  2. Detection of IκB Degradation Dynamics and IκB-α Ubiquitination

  3. Measurement of NF-κB Transcriptional Activity and Identification of NF-κB cis-regulatory Elements using Luciferase Assays

  4. Assessing Sites of NF-κB DNA Binding using Chromatin Immunoprecipitation

  5. IKK Kinase Assay for Assessment of Canonical NF-κB Activation in Neurons

  6. Analysis of Epidermal Growth Factor-induced NF-κB Signaling

  7. Methods to Assess the Activation of the Alternative (non-canonical) NF-κB Pathway by Non-death TNF Receptors

  8. Systematic Detection of Non-canonical NF-κB Activation

  9. Non-canonical NF-κB Activation and SDF-1 Expression in Human Endothelial Cells

  10. Stable Reconstitution of IKK-deficient Mouse Embryonic Fibroblasts

  11. Dissecting NF-κB Signaling Induced by Genotoxic Agents via Genetic Complementation of NEMO-deficient 1.3E2 Cells

  12. Visualizing TCR Induced POLKADOTS Formation and NF-κB Activation in the D10 T Cell Clone and Mouse Primary Effector T Cells

  13. Detection of Recombinant and Cellular MALT1 Paracaspase Activity

  14. TRAF Protein Function in Non-Canonical NF-κB Signaling

  15. Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-κB Signaling

  16. Elucidating Dynamic Protein-Protein Interactions and Ubiquitination in NF-κB Signaling Pathways

  17. Immunoblot Analysis of Linear Polyubiquitination of NEMO

  18. In Vitro Detection of NEMO-Ubiquitin Binding using DELFIA and Microscale Thermophoresis Assays

  19. Use of Fluorescence Spectroscopy for Quantitative Investigations of Ubiquitin Interactions with the Ubiquitin-Binding Domains of NEMO

  20. Generation of a Proteolytic Signal: E3/E2-mediated Polyubiquitination of IκBα

  21. Control of NF-κB Subunits by Ubiquitination

  22. Methodology to Study NF-κB/RelA Ubiquitination in Vivo

  23. Using Sequential Immunoprecipitation and Mass Spectrometry to Identify Methylation of NF-κB

  24. Methods to Detect NF-κB Acetylation and Methylation

  25. A Method for the Quantitative Analysis of Stimulation-Induced Nuclear Translocation of the p65 Subunit of NF-κB from Patient-Derived Dermal Fibroblasts

  26. Methods for Assessing the In Vitro Transforming Activity of NF-κB Transcription Factor c-Rel and Related Proteins

  27. Using RNA Interference in Lung Cancer Cells to Target the IKK-NF-κB Pathway

  28. Immunohistochemical Analysis of NF-κB in Human Tumor Tissue

  29. Assessment of Canonical NF-κB Activity in Canine Diffuse Large B-Cell Lymphoma

  30. NEMO Binding Domain Peptide Inhibition of Inflammatory Signal-Induced NF-κB Activation In Vivo

  31. Regulation of NF-κB Signaling in Osteoclasts and Myeloid Progenitors

  32. NF-κB Activation with Aging: Characterization and Therapeutic Inhibition

  33. The “Sneaking-Ligand” Approach: Cell-Type Specific Inhibition of the Classical NF-κB Pathway

  34. Sneaking-Ligand Fusion Proteins Attenuate Serum Transfer Arthritis by Endothelium-Targeted NF-κB Inhibition

  35. Analysis of NF-κB Activation in Mouse Intestinal Epithelial Cells

  36. Characterizing the DNA Binding Site Specificity of NF-κB with Protein Binding Microarrays (PBMs)

  37. Methods for Analyzing the Evolutionary Relationship of NF-κB Proteins Using Free, Web-driven Bioinformatics and Phylogenetic Tools

  38. Studying NF-κB Signaling with Mathematical Models

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Tags: Michael May, kappa, Methods, Protocols